| 12499120 |
Farina M, Brandao R, de Lara FS, Pagliosa LB, Soares FA, Souza DO, Rocha JB: Profile of nonprotein thiols, lipid peroxidation and delta-aminolevulinate dehydratase activity in mouse kidney and liver in response to acute exposure to mercuric chloride and sodium selenite. Toxicology. 2003 Mar 3;184(2-3):179-87.
The effects of mercury (Hg (2+)) and selenite (Se (4+)) on delta-aminolevulinic acid dehydratase (delta-ALA-D) activity, 2-thiobarbituric acid reactive substances (TBARS) and nonprotein sulfhydryl content (NPSH) in mouse kidney and liver were investigated. Male mice were given a single i.p. injection of Hg (2+) and/or Se (4+) (25 micromol/kg) and were killed at 6, 12, 24 and 48 h after treatment. Hg (2+) inhibited renal delta-ALA-D at 6 and 12 h after treatment. Se (4+) abolished the inhibitory effect of mercury on renal delta-ALA-D at 12 h after treatment. Renal and hepatic NPSH content decreased after Hg (2+) exposure and selenite inhibited, at least in part, the Hg-induced oxidation of renal and hepatic NPSH. Se (4+) and Hg (2+), when injected alone, did not alter hepatic or renal TBARS levels; however, simultaneous exposure to these compounds increased hepatic and renal TBARS levels at 12 and 48 h after treatment, respectively. Present results suggest that selenium abolishes the interaction of Hg (2+) with sulfhydryl groups of protein and nonprotein sources. |
7(0,0,1,2) |