| 10516274 |
Yanagawa N, Pham C, Shih RN, Miao S, Jo OD: Chloride dependency of renal brush-border membrane phosphate transport. . Am J Physiol. 1999 Oct;277(4 Pt 2):F506-12.
In our present study, we examined the effect of Cl (-) on rabbit renal brush-border membrane (BBM) phosphate (P (i)) uptake. It was found that the Na (+)-dependent BBM (32) P uptake was significantly inhibited by Cl (-) replacement in the uptake solution with other anions, or by Cl (-) transport inhibitors, including DIDS, SITS, diphenylamine-2-carboxylate (DPC), niflumic acid (NF), and 5-nitro-2-(3-phenylpropylamino) benzoate (NPPB). Intravesicular formate or Cl (-) increased BBM (36) Cl (-) uptake but did not affect BBM (32) P uptake. BBM (22) Na (+) uptake was lowered by Cl (-) replacement in the uptake solution but not by Cl (-) transport inhibitors. Changes in transmembrane electrical potential altered BBM (36) Cl (-) and (32) P uptake in directions consistent with a net inward movement of negative and positive charges, respectively. However, the Cl (-)-dependent BBM P (i) uptake was not affected by changes in transmembrane electrical potential. Finally, a similar Cl (-) dependency of P (i) uptake was also found with BBM derived from rat and mouse kidneys. In summary, our study showed that a component of Na (+)-dependent P (i) uptake was also Cl (-) dependent in rabbit, rat, and mouse renal BBM. The mechanism underlying this Cl (-) dependency remains to be identified. |
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