| 14695481 |
Xing Y, He Q, Zhu JX, Chan HC: Basolateral membrane mechanisms involved in ligustrazine-stimulated anion secretion in rat distal colon. Sheng Li Xue Bao. 2003 Dec 25;55(6):653-7.
The present study investigated the cellular mechanism underlying the effect of ligustrazine on the ion transport in rat distal colon using the short-circuit current (I (SC)) technique. In freshly isolated colonic strips, basolateral addition of ligustrazine stimulated a rise in I (SC), which was resistant to basolateral application of neuronal sodium channel blocker tetrodotoxin (TTX), but inhibited by 55.2% by basolateral pretreatment with prostaglandin inhibitor indomethacin. The ligustrazine-induced I (SC) increase was inhibited by apical application of Cl (-) channel blockers diphenylamine-2,2'-dicarboxylic acid (DPC) and glibenclamide. Basolaterally administered bumetanide, an inhibitor of Na (+)-K (+)-2 Cl (-) cotransporter, inhibited ligustrazine-evoked current increases by 85.2% and basolateral exposure to Ba (2+), a non-specific potassium channels blocker, and blocked the current by more than 90%, indicating that basolateral Na (+)-K (+)-2 Cl (-) cotransporter and K (+) channels played an important role in the effect of ligustrazine. The results suggested that ligustrazine could stimulate rat distal colon (-) secretion that is mediated by basolateral Na (+)-K (+)-2 Cl (-) cotransporter and K (+) channel. |
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