| 10772756 |
Pushkareva MY, Janoff AS, Mayhew E: Inhibition of cell division but not nuclear division by 1-O- octadecyl-2-O-methyl-Sn-glycero-3-phosphocholine. Cell Biol Int. 1999;23(12):817-28.
1-O-Octadecyl-2-O-methyl-glycero-3-phosphocholine (ET-18-OCH (3)) selectively inhibits the growth of cancer cells. Here we show that in some cell types ET-18-OCH (3) and liposome-associated ET-18-OCH (3) inhibit cell division without concurrent inhibition of nuclear division, leading to multinucleate cell formation, and cell death through apoptosis. Cell cycle analysis revealed that ET-18-OCH (3)-treated U-937 cells continued to move through the cell cycle, but many cells were not able to divide and instead accumulated as tetraploid cells or octaploid cells in the G0/G1 phase of the cell cycle. Inhibition of cytokinesis has been shown to be paralleled by activation of U-937 cells, including upregulation of some cell-surface markers, acquisition of phagocytic activity, and secretion of tumor necrosis factor (TNF)-alpha (Pushkareva et al., 2000). Furthermore, treatment of cells with ET-18-OCH (3) results in the accumulation of apoptotic cells in time- and dose-dependent manner. It is possible that inhibition of cytokinesis may be related to cytoskeletal effects. |
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