| 16774759 |
Hantash J, Bartlett A, Oldfield P, Denes G, O'Rielly R, Roudiere D, Menduni S: Use of an on-line imprinted polymer pre-column, for the liquid chromatographic-UV absorbance determination of carbaryl and its metabolite in complex matrices. J Chromatogr A. 2006 Aug 25;1125(1):104-11. Epub 2006 Jun 13.
A carbaryl and 1-naphtol molecular imprinted polymers (MIP1 and MIP 2, respectively) were prepared using suspension polymerization and tested for selective and reversible binding to carbaryl and 1-naphtol, respectively. In the suspension polymerization technique used, polymers of methacrylic acid (MAA), highly cross-linked with ethylene dimethacrylate, provide a specific binding sites for the carbaryl molecule and its metabolite when using it as a template to be removed after polymerization. The molecular imprinted polymer with a particle size of approximately 5 microm were isolated and packed into a pre-column (50 mm x 4.6 mm id) that was used to isolate carbaryl and its metabolite from complex matrices injected on a high performance liquid chromatography system using ultra-violet detection without extensive sample preparation and clean up. The HPLC method had a detection limit of 1.00 ng/mL and a linear response (r2 > 0.98) over the concentration range of 1.00-10.0 ng/mL. |
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