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Olajos EJ, Rosenblum I: Membrane-bound and soluble esterase activities in various hen brain regions after diisopropyl phosphorofluoridate and trichlorfon treatment. Neurotoxicology. 1981 Nov;2(3):463-70.
Acetylcholinesterase, neurotoxic esterase, and nonspecific esterase activities were studied in subcellular fractions obtained from homogenates of forebrain, cerebellum, and brainstem areas of hen brain. The hens were given a single, oral dose (1.0 mg/kg of Dyflos (diisopropyl phosphorofluoridate) or 100 mg/kg of trichlorfon (dimethyl -2,2,2-trichloro-1-hydroxyethylphosphonate). The animals were killed 24 hr after dosing. The level of neurotoxic esterase and soluble nonspecific esterase activities were consistent from region to region; however, acetylcholinesterase activity among the brain regions varied significantly. Both organophosphorus compounds produced definite inhibition of acetylcholinesterase. The cerebellar acetylcholinesterase activity was the most susceptible to organophosphorus ester-induced inhibition. A marked inhibition of neurotoxic esterase (70-80%) was produced by diisopropyl phosphorofluoridate in all areas. Trichlorfon produced little or no inhibition of neurotoxic esterase (0-10%). Little variation in soluble nonspecific esterase activity (alpha -naphthyl acetate) was noted between the three brain regions studied. Both diisopropyl phosphorofluoridate and trichlorfon failed to significantly alter soluble nonspecific esterase activity among the brain regions. |
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