| 16347784 |
Pipke R, Amrhein N: Isolation and Characterization of a Mutant of Arthrobacter sp. Appl Environ Microbiol. 1988 Nov;54(11):2868-2870.
Strain GLP-1 Which Utilizes the Herbicide Glyphosate as Its Sole Source of Phosphorus and Nitrogen.. Arthrobacter sp. strain GLP-1, grown on glucose as a carbon source, utilizes the herbicide glyphosate [N-(phosphonomethyl) glycine] as its sole source of phosphorus as well as its sole source of nitrogen. The mutant strain GLP-1/Nit-1 utilizes glyphosate as its sole source of nitrogen as well. In strain GLP-1, P (i) was a potent competitive inhibitor of glyphosate uptake (K (i), 24 muM), while the affinity of P (i) for the uptake system of strain GLP-1/Nit-1 was reduced by 2 orders of magnitude (K (i), 2.3 mM). It is concluded that the inability of strain GLP-1 to utilize glyphosate as a source of nitrogen is due to the stringent control of glyphosate uptake by excess phosphate released during the degradation of the herbicide. |
87(1,1,2,2) |