| 18967431 |
Turiel E, Fernandez P, Perez-Conde C, Gutierrez AM, Camara C: Flow-through fluorescence immunosensor for atrazine determination. Talanta. 1998 Dec;47(5):1255-61.
A new flow-through fluoroimmunosensor for atrazine determination based on the use of protein A immobilized on controlled pore glass as immunoreactor is reported. The support, placed in the optical path of the flow cell, allows the 'in situ' quantification of atrazine by on-line antigen-antibody binding upon successive injections of both substances. The immunosensor has a detection limit of 2.1 mug l (-1), a sample speed of about 10 samples per hour, and provides high reproducibility both within-day (3.2% for 5 mug l (-1) and 2.2% for 30 mug l (-1)) and between days. The optimum working concentration range was 2.1-50 mug l (-1). Possible interferences of other triazines like simazine, desethylatrazine (DEA) and desisopropylatrazine (DIA) were evaluated. Simazine and DIA were not cross-reactive; however, the cross-reactivity for DEA was CR=7.7%. The proposed immunosensor was successfully applied to the determination of atrazine in drinking water and citrus fruits. |
6(0,0,1,1) |