| 19075818 |
Mondal P, Ray M, Sahu S, Sabat SC: Co-expression of GroEL/ES enhances the expression of plant catalase in bacterial cytosol. Protein Pept Lett. 2008;15(10):1075-8.
Expression of plant proteins in E. coli is frequently unsuccessful, but soluble and functional rice catalase-B can be produced in E. coli when it is co-expressed with the chaperone GroEL/ES. The rice catalase exhibited properties typical for a catalase including the decomposition of H (2) O (2) and inhibition by aminotriazole, a specific inhibitor for plant and animal catalases. This achievement records for first time the successful expression of a both native and variant rice plant catalase in bacterial cytosol suggesting that it may be an option to be considered for the expression of other plant proteins in E. coli. |
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