| 6530448 |
Wisneski HH, Yates RL, Davis HM: High-performance liquid chromatographic-fluorometric determination of cinnamaldehyde in perfume, cologne and toilet water. J Chromatogr. 1984 Dec 28;317:421-6.
A high-performance liquid chromatographic (HPLC)-fluorometric method is described for the determination of trans-cinnamaldehyde in fragrances. The fragrance is added to isooctane and extracted with an aqueous solution of the sodium salt of 6-aminocaproic acid to isolate the aldehyde fraction. After dilution with water, an aliquot of the extract is added to a solution of 1,2-diaminonaphthalene monosulfate in dilute formic acid. The fluorescent derivative of cinnamaldehyde, 2-styrylnaphth [1,2-d] imidazole, is prepared by incubating and then cooling the solution and adding pyridine. Aliquots of the fluorophore solution are analyzed on a reversed-phase C18 HPLC column by using a buffered tetrahydrofuran-water eluent. Cinnamaldehyde is quantitated by comparing fluorescence emission intensity with that of a standard. Recoveries from samples of various commercial fragrances, spiked with cinnamaldehyde at the 0.01, 0.05 and 0.1% levels, ranged from 94 to 112% with a mean of 103% and a standard deviation of 5.3. The limit of detection is approximately 1 ng. |
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