Protein Information

ID 84
Name aspartate aminotransferase
Synonyms Aspartate Aminotransferase 1; GIG18; GOT 1; GOT1; Aspartate aminotransferase; Glutamate oxaloacetate transaminase 1; Transaminase A; Aspartate aminotransferases…

Compound Information

ID 1225
Name acrolein
CAS 2-propenal

Reference

PubMed Abstract RScore(About this table)
12365456 Southwell JL, Yeargans GS, Kowalewski C, Seidler NW: Acrolein modifies and inhibits cytosolic aspartate aminotransferase. . J Enzyme Inhib Med Chem. 2002 Feb;17(1):19-23.
Acrolein is a reactive lipid peroxidation byproduct, which is found in ischemic tissue. We examined the effects of acrolein on cytosolic aspartate aminotransferase (cAAT), which is an enzyme that was previously shown to be inhibited by glycating agents. cAAT is thought to protect against ischemic injury. We observed that acrolein cross-linked cAAT subunits as evidenced by the presence of high molecular weight bands following SDS-PAGE. Acrolein-modified cAAT resisted thermal denaturation when compared with native cAAT. We also observed a decrease in intrinsic fluorescence (290 nm, ex; 380 nm, em). These observations are consistent with an acrolein-induced change in conformation that is more rigid and compact than native cAAT, suggesting that intramolecular cross-links occurred. Acrolein also inhibited activity, and the inhibition of enzyme activity correlated with the acrolein-induced formation of cAAT cross-links.
112(1,2,2,2)