| 12132692 |
dos Passos Lemos A, Fonseca de Souza AL, de Sa Pinheiro AA, de Berredo-Pinho M, Meyer-Fernandes JR: Ecto-phosphatase activity on the cell surface of Crithidia deanei. Z Naturforsch C. 2002 May-Jun;57(5-6):500-5.
In the present work we have partially characterized an ecto-phosphatase activity in Crithidia deanei, using viable parasites. This enzyme hydrolyzed p-nitrophenylphosphate at a rate of 3.55 +/- 0.47 nmol Pi/h x 10 (8) cells. The dependence on p-NPP concentration shows a normal Michaelis-Menten kinetics for this phosphatase activity and the value of the apparent Km for p-NPP was 5.35 +/- 0.89 mM. This phosphatase activity was inhibited by the product of the reaction, the inorganic phosphate. Experiments using classical inhibitors of acid phosphatases, such as ZnCl2 and sodium fluoride, as well as inhibitors of phosphotyrosine phosphatase, such as sodium orthovanadate and ammonium molybdate, showed a decrease in this phosphatase activity, with different patterns of inhibition. |
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