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Jian L, Chen Q: [Effect of carbon disulfide on pyrogallol- luminol auto-oxidation chemiluminescence system and superoxide anion]. Zhonghua Yu Fang Yi Xue Za Zhi. 2000 May;34(3):162-4. OBJECTIVE: To study the effect of carbon disulfide (CS (2)) on pyrogallol-luminol auto-oxidation chemiluminescence (PA-L) system and production of superoxide anion. METHODS: Luminescence dynamic curve of CS (2) and inhibition effect of superoxide dismutase (SOD) on intensity of chemiluminescence induced by CS (2) were studied with PA-L chemiluminescence system. RESULTS: Different concentrations of CS (2) (10, 40, 80 mg/ml) could enhance the emission intensity of chemiluminescence of PA-L system and delayed its peak time, with a significant dose-effect relationship, as compared with that of solvent ethanol. Peak of chemiluminescence induced by 80 mg/ml of CS (2) was higher than its background value without solvent ethanol. Chemiluminescence induced by CS (2) could be inhibited by SOD. CONCLUSION: CS (2) can generate superoxide anion in PA-L system and delay the peak time of chemiluminescence emission dynamic curve. |
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