| 17362637 |
Lu CH, Lu JX, Hua GP, Zhu J, Wang H, Huang JF, Gu MZ, Zhou Q, Ni RZ: [Effects of antisense RNA of connective tissue growth factor expressing plasmid on rat liver fibrosis]. Zhonghua Gan Zang Bing Za Zhi. 2007 Feb;15(2):118-21.
OBJECTIVE: To observe the effects of antisense RNA of connective tissue growth factor (CTGF) on rat liver fibrosis. METHODS: Gene recombinant techniques were used to construct a rat antisense RNA of CTGF recombinant plasmid which could be expressed in eukaryotic cells. The recombinant plasmids were encapsulated with lipofectamin and then transducted into a carbon tetrachloride (CCl4) induced rat liver fibrosis model. Expression of CTGF was assessed by RT-PCR, Western blot and immunohistochemistry. Immunohistochemistry was used to identify type I and III collagens. HE stained liver slides were used for pathological study. RESULTS: The mRNA and protein expression of CTGF in the fibrotic liver transfected with antisense-CTGF were significantly decreased compared with those of the controls (P <0.01). The depositions of type I and type III collagens were also decreased (P <0.05). Antisense-CTGF also minimized the pathological fibrosis in the rat livers (P <0.01). CONCLUSION: The results demonstrate that the antisense RNA of CTGF recombinant plasmid has certain effects in preventing liver fibrosis and makes it a possible candidate for use in future gene therapy. |
7(0,0,0,7) |