| 20178458 |
Rabani V, Shahsavani M, Gharavi M, Piryaei A, Azhdari Z, Baharvand H: Mesenchymal stem cell infusion therapy in carbon tetrachloride-induced liver fibrosis model affects matrix metalloproteinase expression. Cell Biol Int. 2010 Feb 23.
In order to investigate the effects of bone marrow-derived mesenchymal stem cells (MSCs) in reversing liver fibrosis and to determine its possible mechanism of action, mouse MSCs were infused into the tail vein of a CCl4 injection mouse chronic model. MSCs caused a decrease in liver fibrosis histopathologically four weeks after transplantation. The reduction in liver collagen was confirmed by quantitative analysis. Moreover, lipid peroxidation in the CCl4/MSC group decreased significantly. Quantitative RT-PCR analysis showed administration of MSCs has a significant antifibrotic effect as evidenced by the decrease in expression of liver collagen and increase in matrix metalloproteinase 13 (MMP13) in the CCl4/MSC group when compared to the CCl4 group, four weeks after transplantation. The expression of alphaSMA (smooth muscle actin) and TIMP1 was also downregulated in the CCl4/MSC group. Additionally, the expression of MMP9 significantly upregulated in the CCl4 treated group; however, there was no significant change after MSC injection. Few engrafted cells in the recipient liver and were able to differentiate into albumin-positive cells. In conclusion, MSCs can enhance recovery of a CCl4-injured mouse liver through their influence in reducing collagen deposition by possibly affecting expression of MMPs. |
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