Protein Information

ID 2553
Name surfactant protein B
Synonyms 18 kDa pulmonary surfactant protein; 6 kDa protein; PSP B; Pulmonary surfactant apoprotein PSP B; Pulmonary surfactant associated proteolipid SPL(Phe); Pulmonary surfactant associated protein B; Pulmonary surfactant associated protein B precursor; SFTB 3…

Compound Information

ID 1402
Name methylene chloride
CAS dichloromethane

Reference

PubMed Abstract RScore(About this table)
8747480 van Eijk M, De Haas CG, Haagsman HP: Quantitative analysis of pulmonary surfactant proteins B and C. Anal Biochem. 1995 Dec 10;232(2):231-7.
A procedure for the quantification of surfactant protein B (SP-B) and surfactant protein C (SP-C) in surfactant preparations is described. After butanol extraction of surfactant, the resulting extract is separated by Sephadex LH-60 HPLC, using an elution fluid which consists of 30% dichloromethane, 65% methanol, and 5% 100 mM HCL. SP-B and SP-C are detected by absorbance at 228 nm and quantified using standard stock solutions. Run length is approximately 70 min. Detection limits are 1 microgram SP-B and 4 micrograms SP-C.		 3(0,0,0,3)