| 19023966 |
Dukhanina EA, Romanova EA, Dukhanin AS, Kabanova OD, Lukyanova TI, Shatalov YV, Yashin DV, Gnuchev NV, Sashchenko LP: Interactions and possible functional characteristics of Tag7-S100A4 protein complex. Bull Exp Biol Med. 2008 Feb;145(2):191-3.
Peptidoglycane-recognizing protein Tag7 formed a complex with S100A4 (a representative of S100 protein family), the apparent dissociation constants in the absence and presence of Ca2+ were 2 x l0 (-8) M and 10 (-9) M, respectively. Analysis of fluorescence spectra of hydrophobic fluorescent probe 2-toluidinyl naphthalene-6-sulfonate in the presence of S100A4 and Tag7 proteins showed that extensive area or several sites are involved into the complex formation between these proteins. The formation of Tag7-S100A4 complex had virtually no effect on the role of S100A4 in the regulation of intracellular Ca2+ metabolism. Removal of not only Tag7, but also S100A4 from neutrophil conditioned medium reduced lysis of E. coli cell, while addition of the Tag7-S100A4 complex to the medium restored antibacterial activity. |
33(0,1,1,3) |