Protein Information

ID 1097
Name MutS
Synonyms DNA mismatch repair protein Msh3; MRP1; MutS; DUC 1; DUC1; DUG; DUP; Divergent upstream protein…

Compound Information

ID 1403
Name naphthalene
CAS naphthalene

Reference

PubMed Abstract RScore(About this table)
16876990 Cho M, Chung S, Heo SD, Ku J, Ban C: A simple fluorescent method for detecting mismatched DNAs using a MutS-fluorophore conjugate. Biosens Bioelectron. 2007 Feb 15;22(7):1376-81. Epub 2006 Jul 31.
A fluorescent method was developed for the detection of unpaired and mismatched DNAs using a MutS-fluorophore conjugate. The fluorophore, 2-(4'-(iodoacetoamido) anilino) naphthalene-6-sulfonic acid (IAANS), was site-specifically attached to the 469 position of Thermus aquaticus (Taq.) MutS mutant (C42A/T469C). The fluorophore labeled residue located at the dimer interface of the protein undergoes a drastic conformational change upon binding with mismatched DNA. The close proximity of the two identical fluorescent molecules presumably causes the self-quenching of the fluorophore, since fluorescence emission of the biosensor decreases with increasing concentrations of mismatched DNA. The order of binding affinity for each unpaired and mismatched DNA obtained by this method was DeltaT (Kd=52 nM)> GT (62 nM)> DeltaC (130 nM)> CT (160 nM)> DeltaG (170 nM)> DeltaA (250 nM)> CC (720 nM)> AT (950 nM). This order is comparable to the previous results of the gel mobility shift assay. Thus, this method can be a simple, useful tool for elucidating the mechanism of DNA mismatch repair as well as a novel probe for detecting of genetic mutation.
83(1,1,1,3)