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Peng ZG, Hidajat K, Uddin MS: Adsorption and desorption of lysozyme on nano-sized magnetic particles and its conformational changes. Colloids Surf B Biointerfaces. 2004 Jun 1;35(3-4):169-74.
Adsorption and desorption of lysozyme on nano-sized magnetic particles and its conformational change were studied in this work. Adsorption of lysozyme on nano-sized magnetic particles (Fe (3) O (4)) was carried out at different pH. Maximum adsorption of lysozyme (4.65 mg/m2) occurred at its isoelectric point (pI = 11.1). Differential scanning calorimetry (DSC) results show that the lysozyme adsorbed on magnetic particles did not show any thermal transition over the range 20-100 degrees C. High desorption of lysozyme from magnetic particles was achieved using NaH (2) PO (4) (pH 4.0) (90%) and NaSCN (pH 6.0) (97%) as desorbents. The conformational change of the lysozyme desorbed by NaH (2) PO (4) was small, while the lysozyme desorbed by NaSCN underwent a significant conformational change as measured by the intrinsic fluorescence. Eighty-eight and 82% activity was retained in the desorbed enzyme for desorption by NaH (2) PO (4) and NaSCN, respectively. |
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