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Hernandez-Dominguez E, Campos-Tamayo F, Vazquez-Flota F: Vindoline synthesis in in vitro shoot cultures of Catharanthus roseus. Biotechnol Lett. 2004 Apr;26(8):671-4. Vindoline, the major alkaloid in cultures of Catharanthus roseus shoots, reached 2 mg g (-1) dry wt after 27 d in culture. Maximal vindoline accumulation coincided with maximum activities of deacetoxyvindoline 4-hydroxylase, deacetylvindoline acetyl-CoA acetyl transferase and tryptophan decarboxylase. Shoot exposure to jasmonate shortened the time required for the maximal vindoline accumulation to 14 d. |
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