| 18680725 |
Kim KJ, Lim JH, Kim MJ, Kim T, Chung HM, Paek KH: GDSL-lipase1 (CaGL1) contributes to wound stress resistance by modulation of CaPR-4 expression in hot pepper. Biochem Biophys Res Commun. 2008 Oct 3;374(4):693-8. Epub 2008 Aug 3.
A full length cDNA clone encoding Capsicum annuum GDSL-lipase 1 (CaGL1) was isolated by microarray analysis. The expression of CaGL1 was triggered by methyl jasmonic acid (MeJA), an important signal in abiotic/biotic stress response. However, the expression of this gene was not increased by the application of salicylic acid (SA) or ethylene treatment. And, local/systemic wounding stimuli resulted in rapid accumulation of CaGL1 mRNA. However, CaGL1 was not specifically induced during the hypersensitive response upon Tobacco mosaic virus (TMV) inoculation. By using a virus-induced gene silencing (VIGS)-based reverse genetic approach, it was observed that the suppression of CaGL1 attenuates the expression of Capsicum annuumpathogenesis-related protein 4 (CaPR-4) during wound stress. However, the CaPR-4 transcript level induced by TMV was not regulated by CaGL1 expression. These results indicate that CaGL1 may be involved in signaling pathway of MeJA and/or the wound responses through CaPR-4 expression modulation. |
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