| 16666477 |
Hallahan DL, Heasman AP, Grossel MC, Quigley R, Hedden P, Bowyer JR: Synthesis and Biological Activity of an Azido Derivative of Paclobutrazol, an Inhibitor of Gibberellin Biosynthesis. Plant Physiol. 1988 Dec;88(4):1425-1429.
A photolabile azido derivative of the kaurene oxidase inhibitor 1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-l-yl) pentan-3-ol (paclobutrazol) has been synthesized for use as a photoaffinity labeling agent. The compound was tested as an inhibitor of the oxidation of ent-kaurene catalyzed by cell-free preparations from endosperm of Cucurbita maxima. The I (50) of the azido derivative was 9.5 nanomolar, which compares well with that of paclobutrazol (6.3 nanomolar in our measurements). The azido compound bound to Cytochrome P-450 in microsomes from Cucurbita maxima, and induced a Type II spectral change, with an apparent binding constant of 0.24+/-0.04 micromolar. |
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