| 20149779 |
Park EJ, Na DH: Microchip electrophoresis for monitoring covalent attachment of poly (ethylene glycol) to proteins. Anal Biochem. 2010 May 15;400(2):304-6. Epub 2010 Feb 10.
A microchip electrophoretic method was applied to monitor and characterize the covalent attachment of poly (ethylene glycol) (PEGylation) of two proteins, alpha-lactalbumin and bovine serum albumin, using several poly (ethylene glycol) (PEG) derivatives with molecular weights from 1 to 20 kDa. This method effectively separated multi-PEGylated proteins in a size-based manner and allowed monitoring of the PEGylation pattern with the advantages of high speed, minimal sample consumption, and high reproducibility. Microchip electrophoresis would be a very useful tool for protein PEGylation studies such as reaction monitoring, purity checks, and characterization of PEGylated protein products. |
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