| 14989895 |
He J, Chen JF, Liu R, Wang YB, Wang XR: [Effect of fenvalerate on the ovarian calcium homeostasis and the serum steroid hormone]. Zhonghua Yu Fang Yi Xue Za Zhi. 2004 Jan;38(1):18-21.
OBJECTIVE: To observe the effects of fenvalerate (Fen) on ovarian calcium homeostasis. METHODS: hGLCs were obtained from pre-ovulatory follicles in an in vitro fertilization program, and were cultured for 72 hours. Changes in cellular [Ca (2+)] i induced by Fen in hGLCs were detected with laser scanning confocal microscopy (LSCM) by using the fluorescent Ca (2+) indicator fluo-3/AM. SD female rats were divided into four groups (control, 1/15LD (50), 1/50 LD (50) and 1/250 LD (50)) in experiment. The activity of ovarian Ca (2+)-ATPase and phosphorylase A (P-a) and the contents of calmodulin (CaM) were assessed after a 30-day Fen exposure. In addition, serum estradiol-17 beta (E (2)) and progesterone (P (0)) concentration were measured by radioimmunoassay, which the sampling rats were ensured at diestrus stage before killed according to vaginal smear. RESULTS: 20.0 and 2.0 micromol/L Fen induced the increased of [Ca (2+)] i in hGLC. This [Ca (2+)] i increase mostly resulted from Ca (2+) influx in the studied concentration. Fen had shown the inhibition effects on activity of Ca (2+)-ATPase in 1/250 LD (50) group (P < 0.001) while the activity of phosphorylase A (P-a) in treated groups had significantly enhanced than those of in control. The contents of CaM in ovaries were found to be increased in treated groups. E (2) in 1/250 LD (50) group were higher while P (0) in 1/15 LD (50) group were significantly lower (P < 0.05). CONCLUSION: Exposure to Fen interferes the serum steroid hormone concentrations partly through calcium signal pathway. |
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