ID | 1285 |
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Name | pyrophosphatase |
Synonyms | Cytosolic inorganic pyrophosphatase; IOPPP; Inorganic pyrophosphatase; Inorganic pyrophosphatase 1; PP; PP1; PPA 1; PPA1… |
ID | 1714 |
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Name | gibberellic acid |
CAS | (1α,2β,4aα,4bβ,10β)-2,4a,7-trihydroxy-1-methyl-8-methylenegibb-3-ene-1,10-dicarboxylic acid 1,4a-lactone |
PubMed | Abstract | RScore(About this table) |
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12239377 | Swanson SJ, Jones RL: Gibberellic Acid Induces Vacuolar Acidification in Barley Aleurone. Plant Cell. 1996 Dec;8(12):2211-2221. The roles of gibberellic acid and abscisic acid (ABA) in the regulation of vacuolar pH (pHv) in aleurone cells of barley were investigated using the pH-sensitive fluorescent dye 2 [prime],7 [prime]-bis (2-carboxyethyl)-5 (6)-carboxyfluorescein (BCECF). BCECF accumulated in vacuoles of aleurone cells, but sequestration of the dye did not affect its sensitivity to pH. BCECF-loaded aleurone cells retained their ability to respond to both GA3 and ABA. The pHv of freshly isolated aleurone cells is 6.6, but after incubation in the pHv fell to 5.8. The pHv of cells not incubated in hormones or in the presence of ABA showed little or no acidification. The aleurone tonoplast contains both vacuolar ATPase and vacuolar pyrophosphatase, but the levels of pump proteins were not affected by incubation in the presence or absence of hormones. We conclude that affects the pHv in aleurone cells by altering the activities of tonoplast H+ pumps but not the amounts of pump proteins. |
0(0,0,0,0) |