| ID | 100 |
|---|---|
| Name | acid phosphatase |
| Synonyms | ACP 3; PAP; ACP3; ACPP; ACPP protein; Acid phosphatase; Acid phosphatase prostate nirs variant 1; PACP… |
| ID | 1714 |
|---|---|
| Name | gibberellic acid |
| CAS | (1α,2β,4aα,4bβ,10β)-2,4a,7-trihydroxy-1-methyl-8-methylenegibb-3-ene-1,10-dicarboxylic acid 1,4a-lactone |
| PubMed | Abstract | RScore(About this table) |
|---|---|---|
| 16663801 | Jones RL, Carbonell J: Regulation of the Synthesis of Barley Aleurone alpha-Amylase by Gibberellic Acid and Ions. Plant Physiol. 1984 Sep;76(1):213-218. The effects of gibberellic acid (GA (3)) and ions on the production of alpha-amylase and acid phosphatase by isolated aleurone layers of barley (Hordeum vulgare L. cv Himalaya) were studied. Aleurone layers not previously exposed to GA (3) or Ca (2+) show qualitative and quantitative changes in hydrolase production following incubation in either GA (3) or Ca (2+) or both. Incubation in H (2) O or Ca (2+) results in the production of low levels of alpha-amylase or acid phosphatase. The addition of GA (3) to the incubation medium causes a 10- to 20-fold increase in the amounts of these enzymes released from the tissue, and addition of Ca (2+) at 10 millimolar causes a further 8- to 9-fold increase in alpha-amylase release and a 75% increase in phosphatase release. Production of alpha-amylase isoenzymes is also modified by the levels of GA (3) and Ca (2+) in the incubation medium. alpha-Amylase 2 is produced under all conditions of incubation, while alpha-amylase 1 appears only when layers are incubated in GA (3) or GA (3) plus Ca (2+). The synthesis of alpha-amylases 3 and 4 requires the presence of both GA (3) and Ca (2+) in the incubation medium. Laurell rocket immuno-electrophoresis shows that two distinct groups of alpha-amylase antigens are present in incubation media of aleurone layers incubated with both GA (3) and Ca (2+), while only one group of antigens is found in media of layers incubated in GA (3) alone. ions can be substituted for Ca (2+) in increasing hydrolase production, although higher concentrations of Sr (2+) are required for maximal response. We conclude that GA (3) is required for the production of alpha-amylase 1 and that both GA (3) and either Ca (2+) or Sr (2+) are required for the production of isoenzymes 3 and 4 of barley aleurone alpha-amylase. |
82(1,1,1,2) |