| 18538670 |
Itsuki Y, Saeki M, Nakahara H, Egusa H, Irie Y, Terao Y, Kawabata S, Yatani H, Kamisaki Y: Molecular cloning of novel Monad binding protein containing tetratricopeptide repeat domains. FEBS Lett. 2008 Jul 9;582(16):2365-70. Epub 2008 Jun 5.
We have previously reported that Monad, a novel WD40 repeat protein, potentiates apoptosis induced by tumor necrosis factor-alpha (TNF-alpha) and cycloheximide (CHX). By affinity purification and mass spectrometry, we identified RNA polymerase II-associated protein 3 (RPAP3) as a binding protein of Monad. Overexpression of RPAP3 in HEK 293 potentiated caspase-3 activation and apoptosis induced by TNF-alpha and CHX. In addition, knockdown of RPAP3 by RNA interference resulted in a significant reduction of apoptosis induced by TNF-alpha and CHX in HEK293 and HeLa cells. These results raise the possibility that RPAP3, together with Monad, may function as a novel modulator of apoptosis pathway. |
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