| 20078948 |
Zhai N, Zhang Y, Shen YF: [Effect of PIH1D1 on the degradation of its binding protein SNF5] . Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2009 Dec;31(6):756-9. doi: 10.3881/j.issn.1000-503X.2009.06.021.
OBJECTIVE: To explore the effects of PIH1D1 on its binding protein SNF5, a core subunit of the SWI/SNF chromatin remodeling complex. METHOD: The degradation pathway of SNF5 was identified with protein synthesis inhibitor cycloheximide (CHX) and a potent proteasome inhibitor MG132, and then the PIH1D1 eukaryotic expression plasmid was transfected to explore its effect on the stability of SNF5. RESULTS: HEK293T cells were effectively treated with CHX (optimal concentration: 400microg/ml) and MG132 (optimal concentration: 20micromol/L). The degradation of SNF5 was mediated by the proteasome pathway. PIH1D1 regulated the protein level of SNF5 by attenuating its proteasome degradation. CONCLUSION: PIH1D1 may stabilize SNF5 by attenuating its proteasome degradation pathway. |
86(1,1,1,6) |