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Nishiuchi S, Liu S, Takano T: Isolation and characterization of a metallothionein-1 protein in Chloris virgata Swartz that enhances stress tolerances to oxidative, salinity and carbonate stress in Saccharomyces cerevisiae. Biotechnol Lett. 2007 Aug;29(8):1301-5. Epub 2007 May 22.
Chloris virgata Swartz (C. virgata) is a gramineous wild plant that is found in alkaline soil areas in northeast China and is highly tolerant to carbonate stress. We constructed a cDNA library from C. virgata seedlings treated with NaHCO (3), and isolated a type 1 metallothionein (MT1) gene (ChlMT1: AB294238) from the library. The amino acid sequence of ChlMT1 contained 12 cysteine residues that constituted the Cys-X-Cys (X = amino acid except Cys) motifs in the N- and C-terminal regions. Northern hybridization showed that expression of ChlMT1 was induced by several abiotic stresses, from salts (NaCl and NaHCO (3)), a ROS inducer (paraquat), and metals (CuSO (4), ZnSO (4), and CoCl (2)). ChlMT1 expression in leaf was induced by 200 mM NaCl and 100 mM NaHCO (3). About 5 microM Paraquat, 500 microM Zn (2+), and 500 microM Co (2+) also induced expression of ChlMT1 in leaf after 6 h, and 100 microM Cu (2+) induced it after 24 h. Saccharomyces cerevisiae when transformed with the ChlMT1 gene had dramatically increased tolerances to salts (NaCl and NaHCO (3)) and ROS. |
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