| 11811537 |
Benov L, Al-Ibraheem J: Glycerol metabolism in superoxide dismutase-deficient Escherichia coli. Free Radic Res. 2001 Dec;35(6):867-72.
Escherichia coli, which lacks cytoplasmic superoxide dismutases, exhibits various phenotypic deficits if grown aerobically. Here we report that sodAsodB E. coli cannot use glycerol under aerobic conditions. The reason is low activity of glycerol kinase (GK), the rate-limiting enzyme in glycerol metabolism. Superoxide does not inactivate GK, but makes it susceptible to inactivation by a heat-labile factor present in the cell-free extracts. This factor seems to be part of a proteolytic system, which recognizes and degrades oxidatively modified proteins. |
2(0,0,0,2) |