| 20095326 |
Huang M, Zhang P, Chang XL, Gu XA, Wu Q, Zhou ZJ: [Changes of cytokine and nuclear factor-kappa B in acute paraquat poisoned rats]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009 Aug;27(8):463-7.
OBJECTIVE: To observe the changes of cytokine and NF-kappaB activity in acute paraquat poisoned rats and the effect of PDTC and the mechanism of lung injury caused by paraquat poisoning. METHODS: Sprague-Dawley (SD) rats were randomly divided into three groups: Control group (6 rats), PQ group (56 rats) and PQ + PDTC group (46 rats). On the 1st, the 3rd, the 7th, the 14th, the 28th and the 56th day after treatment, the level of interleukin-1 beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), transforming growth factor-beta 1 (TGF-beta1) and platelet-derived growth factor (PDGF) in serum were detected; the activity of NF-kappaB in lung tissues was detected. RESULTS: The level of IL-1beta increased significantly on the 1st, the 3rd, the 7th day in PQ group compared with control group (P <0.01). The content of TGF-beta1 and TNF-alpha in PQ group significantly increased compared with control group (P <0.05 or P <0.01). The level of PDGF significantly increased on the 7th, the 14th, the 28th and the 56th day compared with control group (P <0.01). Meanwhile, IL-1beta and TNF-alpha were positively correlated with lung coefficient (r=0.37, 0.46, P <0.05 or P <0.01 ); TGF-beta1 and PDGF had positive correlation with hydroxyproline (r=0.56, 0.89, P <0.01). The activity of NF-kappaB in lung tissue of PQ group significantly increased on the 1st, the 3rd, the 7th and 14th day compared with control (P <0.05 or P <0.01). There was a significant decrease of IL-1beta, TGF-beta1, TNF-alpha and PDGF in PQ + PDTC group compared with PQ group (P <0.05 or P <0.01) in the corresponding sacrifice dates. There was a significant decrease in NF-kappaB activity on the 1st, the 3rd, the 7th day in PQ + PDTC group compared with PQ group (P <0.01). CONCLUSION: The cytokine and NF-kappaB could play an important role in lung injury of poisoned rats. PDTC may inhibit the expression of NF-kappaB and further reduce the production of cytokines, alleviate lung injury in acute paraquat poisoned rats. |
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