| 17160388 |
Kolla VA, Vavasseur A, Raghavendra AS: Hydrogen peroxide production is an early event during bicarbonate induced stomatal closure in abaxial epidermis of Arabidopsis. Planta. 2007 May;225(6):1421-9. Epub 2006 Dec 8.
The presence of 2 mM bicarbonate in the incubation medium induced stomatal closure in abaxial epidermis of Arabidopsis. Exposure to 2 mM bicarbonate elevated the levels of H (2) O (2) in guard cells within 5 min, as indicated by the fluorescent probe, dichlorofluorescein diacetate (H (2) DCF-DA). Bicarbonate-induced stomatal closure as well as H (2) O (2) production were restricted by exogenous catalase or diphenylene iodonium (DPI, an inhibitor of NAD (P) H oxidase). The reduced sensitivity of stomata to bicarbonate and H (2) O (2) production in homozygous atrbohD/F double mutant of Arabidopsis confirmed that NADP (H) oxidase is involved during bicarbonate induced ROS production in guard cells. The production of H (2) O (2) was quicker and greater with ABA than that with bicarbonate. Such pattern of H (2) O (2) production may be one of the reasons for ABA being more effective than bicarbonate, in promoting stomatal closure. Our results demonstrate that H (2) O (2) is an essential secondary messenger during bicarbonate induced stomatal closure in Arabidopsis. |
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