| 18210155 |
Takasaki H, Mahmood T, Matsuoka M, Matsumoto H, Komatsu S: Identification and characterization of a gibberellin-regulated protein, which is ASR5, in the basal region of rice leaf sheaths. Mol Genet Genomics. 2008 Apr;279(4):359-70. Epub 2008 Jan 22.
Gibberellins (GAs) regulate growth and development in higher plants. To identify GA-regulated proteins during rice leaf sheath elongation, a proteomic approach was used. Proteins from the basal region of leaf sheath in rice seedling treated with GA (3) were analyzed by fluorescence two-dimensional difference gel electrophoresis. The levels of abscisic acid-stress-ripening-inducible 5 protein (ASR5), elongation factor-1 beta, translationally controlled tumor protein, fructose-bisphosphate aldolase and a novel protein increased; whereas the level of RuBisCO subunit binding-protein decreased by GA (3) treatment. ASR5 out of these six proteins was significantly regulated by GA (3) at the protein level but not at the mRNA level in the basal region of leaf sheaths. Since this protein is regulated not only by abscisic acid but also by GA (3), these results indicate that ASR5 might be involved in plant growth in addition to stress in the basal regions of leaf sheaths. |
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