| 12768346 |
Raeymaekers T, Potters G, Asard H, Guisez Y, Horemans N: Copper-mediated oxidative burst in Nicotiana tabacum L. cv. Protoplasma. 2003 May;221(1-2):93-100.
Bright Yellow 2 cell suspension cultures.. In cell suspension cultures of Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) a rapid and concentration-dependent accumulation of H (2) O (2) is induced by excess concentrations of copper (up to 100 microM). This specific and early response towards copper stress was shown to be extracellular. Addition of 300 U of catalase per ml decreased the level of H (2) O (2). Superoxide dismutase (5 U/ml) induced an increase in H (2) O (2) production by 22.2%. This indicates that at least part of the H (2) O (2) is produced by dismutation of superoxide. Pretreatment of the cell cultures with the NAD (P) H oxidase inhibitors diphenylene iodonium (2 and 10 microM) and quinacrine (1 and 5 mM) prevented the generation of H (2) O (2) under copper stress for 90%. The influence of the pH on the H (2) O (2) production revealed the possible involvement of cell-wall-dependent peroxidases in the generation of reactive oxygen species after copper stress. |
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