| 19003289 |
Valle MA, Kester MB, Burns AL, Marx SJ, Spiegel AM, Shiloach J: Production and purification of human menin from Drosophila melanogaster S2 cells using stirred tank reactor. Cytotechnology. 2001 Mar;35(2):127-35.
A process was developed for producing human menin from transformed Drosophila Schneider 2 cells. Protein expression was achieved after inducing the metallothionein promoter by adding copper sulfate to cells growing in suspension in a stirred-tank reactor. Experiments in shake flasks showed that the production of menin was improved when the induction was conducted late in the exponential phase of cell growth at a concentration of 1-2 x 10 (7) cells ml (-1), with a copper concentration of 0.2 mM for no more than 24 h. This observation was confirmed by experiments in bench-scale fermentors. Subsequently, a pilot-scale fermentation yielded 1 mg l (-1) culture of purified menin. |
6(0,0,1,1) |