| 19219608 |
Tougou M, Yamagishi N, Furutani N, Kaku K, Shimizu T, Takahata Y, Sakai J, Kanematsu S, Hidaka S: The application of the mutated acetolactate synthase gene from rice as the selectable marker gene in the production of transgenic soybeans. Plant Cell Rep. 2009 May;28(5):769-76. Epub 2009 Feb 15.
We investigated selective culturing conditions for the production of transgenic soybeans. In this culturing system, we used the acetolactate synthase (ALS)-inhibiting herbicide-resistance gene derived from rice (Os-mALS gene) as a selectable marker gene instead of that derived from bacteria, which interfered with the cultivation and practical usage of transgenic crops. T (1) soybeans grown from one regenerated plant after selection of the ALS-targeting pyrimidinyl carboxy (PC) herbicide bispyribac-sodium (BS) exhibited herbicide resistance, and the introduction and expression of the Os-mALS gene were confirmed by genetic analysis. The selective culturing system promoted by BS herbicide, in which the Os-mALS gene was used as a selectable marker, was proved to be applicable to the production of transgenic soybeans, despite the appearance of escaped soybean plants that did not contain the Os-mALS transgene. |
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