| 7789509 |
Kulaeva ON, Karavaiko NN, Selivankina SYu, Zemlyachenko YaV, Shipilova SV: Receptor of trans-zeatin involved in transcription activation by cytokinin. FEBS Lett. 1995 Jun 5;366(1):26-8.
Zeatin-binding protein (67 +/- 2 kDa) was isolated from the cytosol of the first leaf of 10-day-old barley plants. The protein fits to all requirements for a zeatin receptor: (i) it binds [3H] trans-zeatin reversibly and specifically, (ii) it is recognized by anti-idiotype antibodies from antiserum raised against trans-zeatin, (iii) in concert with 10 (-8) M trans-zeatin it activates rRNA synthesis in vitro in a transcription elongation system containing chromatin from barley leaves associated with RNA-polymerase I. In the presence of trans-zeatin, the protein activates also RNA synthesis directed by RNA-polymerase I and RNA-polymerase II in isolated nuclei from barley leaves. |
81(1,1,1,1) |