| 12505160 |
Laukens K, Lenobel R, Strnad M, Van Onckelen H, Witters E: Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase. FEBS Lett. 2003 Jan 2;533(1-3):63-6.
Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be disrupted by several other adenine- but not guanine-based purine derivatives. The observed interaction with cytokinins is discussed in view of a putative role for adenosine kinase in TBY-2 cytokinin metabolism. The presented results show for the first time a plant adenosine kinase affinity-purified to homogeneity that was identified by primary structure analysis. |
85(1,1,1,5) |