| 11078887 |
Florent I, Mouray E, Dali Ali F, Drobecq H, Girault S, Schrevel J, Sergheraert C, Grellier P: Cloning of Plasmodium falciparum protein disulfide isomerase homologue by affinity purification using the antiplasmodial inhibitor 1,4-bis [3-[N-(cyclohexyl methyl) amino] propyl] piperazine.. FEBS Lett. 2000 Nov 10;484(3):246-52.
A series of 10 1,4-bis (3-aminopropyl) piperazine compounds was found to display antiplasmodial activity with 50% growth inhibition between 30 and 250 nM, on three Plasmodium falciparum strains differently sensitive to chloroquine. By affinity chromatography using one of these compounds, a 52-kDa protein was isolated from P. falciparum, microsequenced and cloned. It corresponded to a single copy gene encoding a 453 amino acid protein displaying the typical features of protein disulfide isomerases, a thiol metabolizing enzyme belonging to the thiol: disulfide oxidoreductase superfamily, which was not previously described in malarial species. |
31(0,1,1,1) |