| 11360653 |
Zhao X, Wei Y, Peng Z: [Phorbol-12-myristate-13-acetate and interferon-gamma synergistic induction of B7-1 expression in ovary carcinoma cells and immunological value]. Zhonghua Fu Chan Ke Za Zhi. 1999 Jul;34(7):414-6.
OBJECTIVE: To investigate the therapeutic potential of phorbol-12-myristate-13-acetate (PMA) and interferon-gamma (IFN-gamma) by induction of expression of adhesion molecule in mucious cystadenocarcinoma of ovary (MCAS) and other tumor cells. METHODS: Expression of costimulatory signals (B7-1), major histocompatibility complex (MHC-I, MHC-II) was analysed with flow cytometry. Cytotoxic T lymphocytes (CTL) killing activity and T cell proliferation induced with expression of B7-1 were evaluated by 4-h 51Cr release assay and standard 3HTdR incorporation and scintitation counting. RESULTS: Pretreatment with PMA and then treated with IFN-gamma was able to enhance B7-1 expression on the tumor cell lines with the constitutive B7-1 expression or on B7-1-transfected tumor cells and to induce B7-1 expression on MCAS and some tumor cell lines without constitutive B7-1 expression. However, treatment with IFN-gamma alone failed to induce B7-1 expression on the tumor cell lines. The synergistic effect of PMA combined with IFN-gamma in the induction of B7-1 expression can be anatagonised by H7 [1-(5-isoquinolinesulfonyl)-2-methyl-Piperazine dihydrochloride]. In addition, treatment with PMA combined with IFN-gamma simultaneously induced or enhanced the expression of MHC class I/II on these tumor cell lines. The tumor cells treated by PMA combined with IFN-gamma were able to induce CTL killing activity and T cell proliferation, which is involved in the expression of B7-1. CONCLUSIONS: The enhancement or induction of B7-1 expression by IFN-gamma may be dependent on the prior activation of protein kinase by PMA and the induced B7-1 expression on tumor cells may have some therapeutic potential. |
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