| 3006586 |
Van Dyk TK, Falco SC, LaRossa RA: Rapid physical mapping by transposon Tn5 mutagenesis to localize the cloned yeast ILV2 gene. Appl Environ Microbiol. 1986 Jan;51(1):206-8.
A rapid method for Tn5 mutagenesis of cloned genes on multicopy plasmids was used to map a yeast ILV2 mutant allele encoding a sulfometuron methyl-resistant acetolactate synthase. Twenty-one of 40 independent Tn5 insertions were within the 5.6-kilobase-pair cloned segment. Of these, seven adjacent transposition events inactivated the sulfometuron methyl resistance determinant, localizing the ILV2 gene to a minimum 1.4-kilobase-pair region. |
6(0,0,1,1) |