GTPase

General Information (Source: NCBI Gene,UniProt)

Text mining Term GTPase
UniProt ID DER_ECOLI
Name GTP-binding protein EngA
Double era-like domain protein
GTPase Der
Gene Names der
Taxonomy Escherichia coli K-12

General annotation

Function GTPase that plays an essential role in the late steps of ribosome biogenesis. GTPase point mutations (but not a deletion mutant) are suppressed by mild overexpression of RelA, probably due to increased levels of the stringent response mediator (p)ppGpp. 50S subunits assembled in the absence of Der are defective and unable to assemble into 70S ribosomes. GTPase activity is stimulated by YihI. Overexpression rescues an rrmJ deletion, stabilizing the 70S ribosome. Der and RrmJ are likely to share a mechanism to stabilize 50S ribosomal subunits at a very late stage of 50S subunit maturation possibly by interacting with 23S rRNA or 23S rRNA/r-protein complex.
Subcellular location

Gene Ontology

GO:0005525 GTP binding MF
GO:0005737 cytoplasm CC
GO:0043022 ribosome binding MF
GO:0003924 GTPase activity MF
GO:0000027 ribosomal large subunit assembly BP

Sequence

>gi|90111450|ref|NP_417006.2| GTPase; multicopy suppressor of ftsJ [Escherichia coli str. K-12 substr. MG1655]
MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGRAEIEGREFICIDTGGIDGTEDGVETR
MAEQSLLAIEEADVVLFMVDARAGLMPADEAIAKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEI
YPIAASHGRGVLSLLEHVLLPWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGR
PNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVIKTLQ
AIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQVKETLDFRLGFIDFARV
HFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAVEDHQPPLVRGRRVKLKYAHAGGYNPPIV
VIHGNQVKDLPDSYKRYLMNYFRKSLDVMGSPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK

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Database Cross Reference

Ensembl Gene
UniGene
PDB
RefSeq NP_417006.2
Pfam PF01926