| Text mining Term | GTPase |
|---|---|
| UniProt ID | DER_ECOLI |
| Name |
Double era-like domain protein GTP-binding protein EngA GTPase Der |
| Gene Names |
der
|
| Taxonomy | Escherichia coli K-12 |
| Function |
GTPase that plays an essential role in the late steps of ribosome biogenesis. GTPase point mutations (but not a deletion mutant) are suppressed by mild overexpression of RelA, probably due to increased levels of the stringent response mediator (p)ppGpp. 50S subunits assembled in the absence of Der are defective and unable to assemble into 70S ribosomes. GTPase activity is stimulated by YihI. Overexpression rescues an rrmJ deletion, stabilizing the 70S ribosome. Der and RrmJ are likely to share a mechanism to stabilize 50S ribosomal subunits at a very late stage of 50S subunit maturation possibly by interacting with 23S rRNA or 23S rRNA/r-protein complex. |
|---|---|
| Subcellular location |
| GO:0005525 | GTP binding | MF |
| GO:0043022 | ribosome binding | MF |
| GO:0003924 | GTPase activity | MF |
| GO:0005737 | cytoplasm | CC |
| GO:0000027 | ribosomal large subunit assembly | BP |
>gi|90111450|ref|NP_417006.2| GTPase; multicopy suppressor of ftsJ [Escherichia coli str. K-12 substr. MG1655] MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGRAEIEGREFICIDTGGIDGTEDGVETR MAEQSLLAIEEADVVLFMVDARAGLMPADEAIAKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEI YPIAASHGRGVLSLLEHVLLPWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGR PNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVIKTLQ AIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQVKETLDFRLGFIDFARV HFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAVEDHQPPLVRGRRVKLKYAHAGGYNPPIV VIHGNQVKDLPDSYKRYLMNYFRKSLDVMGSPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK |
| Ensembl Gene | |
|---|---|
| UniGene | |
| PDB | |
| RefSeq |
NP_417006.2 |
| Pfam |
PF01926 |