Protein Information

Name protein is
Synonyms ANX 2; p36; LIP 2; LIP2; ANX2; ANX2L4; ANX2P1; ANX2P2…

Compound Information

Name TCA
CAS 2,2,2-trichloroacetic acid

Reference List

PubMed Abstract RScore(About this table)
19368890 Barthe P, Roumestand C, Canova MJ, Kremer L, Hurard C, Molle V, Cohen-Gonsaud M: Dynamic and structural characterization of a bacterial FHA protein reveals a new autoinhibition mechanism. Structure. 2009 Apr 15;17(4):568-78.


The OdhI protein is key regulator of the TCA cycle in Corynebacterium glutamicum.
2(0,0,0,2) Details
17101781 Pan Y, Mansfield KD, Bertozzi CC, Rudenko V, Chan DA, Giaccia AJ, Simon MC: Multiple factors affecting cellular redox status and energy metabolism modulate hypoxia-inducible factor prolyl hydroxylase activity in vivo and in vitro. Mol Cell Biol. 2007 Feb;27(3):912-25. Epub 2006 Nov 13.


Importantly, the ODD fusion protein is regulated with kinetics identical to endogenous HIF-1alpha during cellular hypoxia and reoxygenation.
1(0,0,0,1) Details
12456651 Ruprich-Robert G, Zickler D, Berteaux-Lecellier V, Velot C, Picard M: Lack of mitochondrial citrate synthase discloses a new meiotic checkpoint in a strict aerobe. EMBO J. 2002 Dec 2;21(23):6440-51.


Enzyme assays, immunofluorescence and western blotting experiments show that the presence of the mCS protein is more important for completion of meiosis than its well-known enzyme activity.
1(0,0,0,1) Details
20149107 Williams BJ, Du RH, Calcutt MW, Abdolrasulnia R, Christman BW, Blackwell TS: Discovery of an operon that participates in agmatine metabolism and regulates biofilm formation in Pseudomonas aeruginosa. Mol Microbiol. 2010 Feb 10.


Agu2A' contains a twin-arginine translocation signal at its N-terminus and site-directed mutagenesis and cell fractionation experiments confirmed this protein is secreted to the periplasm.
1(0,0,0,1) Details
15975908 Regev-Rudzki N, Karniely S, Ben-Haim NN, Pines O: Yeast aconitase in two locations and two metabolic pathways: seeing small amounts is believing. Mol Biol Cell. 2005 Sep;16(9):4163-71. Epub 2005 Jun 22.


We developed two strategies for physical and functional detection of aconitase in the yeast cytosol: 1) we fused the alpha peptide of the beta-galactosidase enzyme to aconitase and observed alpha complementation in the cytosol; and 2) we created an ACO1-URA3 hybrid gene, which allowed isolation of strains in which the hybrid protein is exclusively targeted to mitochondria.
1(0,0,0,1) Details
18828902 Avall-Jaaskelainen S, Hynonen U, Ilk N, Pum D, Sleytr UB, Palva A: Identification and characterization of domains responsible for self-assembly and cell wall binding of the surface layer protein of Lactobacillus brevis ATCC 8287. BMC Microbiol. 2008 Oct 1;8:165.


This protein is completely unrelated in sequence to the previously characterized S-layer proteins of Lactobacillus acidophilus group.
1(0,0,0,1) Details
17125458 Naeem A, Ashraf MT, Akram M, Khan RH: Comparative study of effects of polyols, salts, and alcohols on trichloroacetic acid-induced state of cytochrome C. Biochemistry. 2006 Oct;71(10):1101-9.


The near- and far-UV CD spectra showed a significant loss of tertiary and secondary structure, although the protein is relatively less unfolded as compared with a conformation at pH 2.0.
1(0,0,0,1) Details
15917633 Carlson E, Bays N, David L, Skach WR: Reticulocyte lysate as a model system to study endoplasmic reticulum membrane protein degradation. Methods Mol Biol. 2005;301:185-205.


Because the newly translated protein is the only source of radiolabel, degradation can be followed to its end state by conversion into trichloroacetic acid (TCA)-soluble peptide fragments.
1(0,0,0,1) Details
18693742 Zhang H, Yang C, Li C, Li L, Zhao Q, Qiao C: Functional assembly of a microbial consortium with autofluorescent and mineralizing activity for the biodegradation of organophosphates. J Agric Food Chem. 2008 Sep 10;56(17):7897-902. Epub 2008 Aug 12.


Green fluorescent protein (GFP) is a stable and easily detectable marker in monitoring genetically engineered microorganisms (GEMs) in the environment.
1(0,0,0,1) Details
15942004 Lefebre MD, Flannagan RS, Valvano MA: A minor catalase/peroxidase from Burkholderia cenocepacia is required for normal aconitase activity. Microbiology. 2005 Jun;151(Pt 6):1975-85.


The authors conclude that the KatA protein is a specialized catalase/peroxidase that has a novel function by contributing to maintain the normal activity of the TCA cycle, while KatB is a classical catalase/peroxidase that plays a global role in cellular protection against oxidative stress.
1(0,0,0,1) Details
18770543 Lovrien R, Matulis D: Assays for total protein. Curr Protoc Microbiol. 2005 Oct;Appendix 3:Appendix 3A.


Acid hydrolysis of a protein is coupled with ninhydrin detection to quantitate amino acid content of a sample.
1(0,0,0,1) Details
20042463 Dallabona C, Marsano RM, Arzuffi P, Ghezzi D, Mancini P, Zeviani M, Ferrero I, Donnini C: Sym1, the yeast ortholog of the MPV17 human disease protein, is a stress-induced bioenergetic and morphogenetic mitochondrial modulator. Hum Mol Genet. 2010 Mar 15;19(6):1098-107. Epub 2009 Dec 30.

1(0,0,0,1) Details
12597597 Lindegardh N, Ashton M, Bergqvist Y: Automated solid-phase extraction method for the determination of piperaquine in plasma by peak compression liquid chromatography. J Chromatogr Sci. 2003 Jan;41(1):44-9.


Protein is precipitated from plasma with acetonitrile-1% aqueous acetic acid (85:15, v/v).
1(0,0,0,1) Details