Protein Information

Name EGFR
Synonyms EGF receptor; EGFR; EGFR protein; ERBB; ERBB 1; ERBB1; Epidermal growth factor receptor; Epidermal growth factor receptor isoform a variant…

Compound Information

Name chloroform
CAS trichloromethane

Reference List

PubMed Abstract RScore(About this table)
19272638 Hayes MP, Douglas W, Ellenson LH: Molecular alterations of EGFR and PIK3CA in uterine serous carcinoma. . Gynecol Oncol. 2009 Jun;113(3):370-3. Epub 2009 Mar 9.

For mutational analysis, neoplastic tissue was microdissected and DNA was extracted with phenol-chloroform.
8(0,0,0,8) Details
17845757 Xu ML, Liu Y, Zhong HH, Wu BQ: [Status and clinicopathologic implication of epidermal growth factor receptor mutation in non-small cell carcinoma of lung]. Zhonghua Bing Li Xue Za Zhi. 2007 Jul;36(7):453-6.

METHOD: DNA was extracted from the excised tumor specimens of 66 non-small cell lung carcinoma patients by traditional phenol-chloroform and ethanol precipitation.
5(0,0,0,5) Details
18985444 Liu Y, Xu ML, Zhong HH, Heng WJ, Wu BQ: EGFR mutations are more frequent in well-differentiated than in poor-differentiated lung adenocarcinomas. Pathol Oncol Res. 2008 Dec;14(4):373-9. Epub 2008 Nov 5.

DNA was extracted from the excised specimens of 60 lung adenocarcinoma patients with phenol-chloroform and ethanol precipitation.
5(0,0,0,5) Details
19424628 Tanaka K, Otake K, Mohri Y, Ohi M, Yokoe T, Toiyama Y, Miki C, Tonouchi H, Kusunoki M: Clinical significance of the gene expression profile in residual tumor cells after neoadjuvant chemo-radiotherapy for esophageal cancer. Oncol Rep. 2009 Jun;21(6):1489-94.

Total RNA was successfully obtained from 6 formalin-fixed paraffin-embedded (FFPE) specimens using proteinase K digestion and phenol chloroform extraction.
TS and DPD as the 5-FU pathway gene, ERCC1 as the CDDP pathway gene, and EGFR, VEGF, HIF1a as radioresistant genes were measured using real-time reverse transcription polymerase chain reaction; comparing the mRNA level of each gene in pre-CRT biopsy with that in post-CRT FFPE specimens.
2(0,0,0,2) Details