Protein Information

Name hexokinase
Synonyms Brain form hexokinase; HK1; Glycolytic enzyme; HK I; HK1 sa; HK1 sb; HK1 ta; HK1 tb…

Compound Information

Name anthraquinone
CAS 9,10-anthracenedione

Reference List

PubMed Abstract RScore(About this table)
12851734 Perez-Ruiz T, Martinez-Lozano C, Tomas V, Martin J: Determination of ATP via the photochemical generation of hydrogen peroxide using flow injection luminol chemiluminescence detection. Anal Bioanal Chem. 2003 Sep;377(1):189-94. Epub 2003 Jul 8.

The method is based on the reaction of glucose with ATP catalyzed by hexokinase and Mg (2+) ions.
The glucose that is not consumed by ATP is subsequently photooxidized using 9,10-anthraquinone-2,6-disulfonate as a sensitizer.
1(0,0,0,1) Details
8198558 Puri RN, Roskoski R Jr: Inactivation of yeast hexokinase by Cibacron Blue 3G-A: spectral, kinetic and structural investigations. Biochem J. 1994 May 15;300 ( Pt 1):91-7.

A comparison of the computer-generated three-dimensional (3D) representations showed considerable overlap of the purine ring of ATP, a nucleotide substrate of hexokinase, with the hydrophobic anthraquinone moiety of the blue dye.
88(1,1,1,8) Details