Protein Information

Name ATPase
Synonyms ATP7A; MK; ATPase; Cation transporting ATPase; ATP7A protein; ATPase Cu(2+) transporting alpha polypeptide; Copper pump 1; Copper transporting ATPase 1…

Compound Information

Name 2,4-D
CAS 2-(2,4-dichlorophenoxy)acetic acid

Reference List

PubMed Abstract RScore(About this table)
14724931 Li S, Xing GM, Cui KR, Yu CH, Zhang X, Xu HX, Wang YF: [Ultracytochemical localization of calcium and ATPase activity on the 2,4-D induced somatic embryogenesis of Lycium barbarum L.]. Shi Yan Sheng Wu Xue Bao. 2003 Dec;36(6):414-20.

In order to research the function mechanism of the 2,4-D during the development of plant somatic embryogenesis, we studied its function mechanism and relationship with the space-time distributing of Ca2+ content and ATPase activity on somatic embryogenesis of Lycium barbarum L.
102(1,1,4,7) Details
11572451 Muller RH, Kleinsteuber S, Babel W: Physiological and genetic characteristics of two bacterial strains utilizing phenoxypropionate and phenoxyacetate herbicides. Microbiol Res. 2001;156(2):121-31.

Strain MC1 has about threefold higher activities for the degradation of dichlorprop and for growth on this substrate (mumax = 0.15 h (-1)) than strain P230; the maximum growth rate on 2,4-D amounts to 0.045 h (-1) with strain MC1.
69(0,2,3,4) Details
16715405 Hoffmann D, Muller RH: 2,4-Dichlorophenoxyacetic acid (2,4-D) utilization by Delftia acidovorans MC1 at alkaline pH and in the presence of dichlorprop is improved by introduction of the tfdK gene. Biodegradation. 2006 Jun;17(3):263-73.

Growth of Delftia acidovorans MC1 on 2,4-dichlorophenoxyacetic acid (2,4-D) and on racemic 2-(2,4-dichlorophenoxy) propanoic acid ((RS)-2,4-DP) was studied in the perspective of an extension of the strain's degradation capacity at alkaline pH.
49(0,1,4,4) Details
9642189 Zipper C, Bunk M, Zehnder AJ, Kohler HP: Enantioselective uptake and degradation of the chiral herbicide dichlorprop [(RS)-2-(2,4-dichlorophenoxy) propanoic acid] by Sphingomonas herbicidovorans MH. J Bacteriol. 1998 Jul;180(13):3368-74.

Transport of (R)-dichlorprop, (S)-dichlorprop, and 2,4-D was completely inhibited by various uncouplers and by nigericin but was only marginally inhibited by valinomycin and by the ATPase inhibitor N,N'-dicyclohexylcarbodiimine.
31(0,1,1,1) Details
14652018 Fernandes AR, Durao PJ, Santos PM, Sa-Correia I: Activation and significance of vacuolar H+-ATPase in Saccharomyces cerevisiae adaptation and resistance to the herbicide 2,4-dichlorophenoxyacetic acid. Biochem Biophys Res Commun. 2003 Dec 26;312(4):1317-24.

The stimulation of the activity of the H (+)-ATPase present in the vacuolar membrane (V-ATPase) of Saccharomyces cerevisiae is here described in response to a moderate stress induced by 2,4-dichlorophenoxyacetic acid (2,4-D).
14(0,0,2,4) Details
16861799 Muller RH, Hoffmann D: Uptake kinetics of 2,4-dichlorophenoxyacetate by Delftia acidovorans MC1 and derivative strains: complex characteristics in response to pH and growth substrate. Biosci Biotechnol Biochem. 2006 Jul;70(7):1642-54.

The present investigation showed that active processes were involved in the uptake of 2,4-dichlorophenoxyacetate (2,4-D) by Delftia acidovorans MC1.
13(0,0,2,3) Details
1647945 Cascorbi I, Foret M: Interaction of xenobiotics on the glucose-transport system and the Na+/K (+)-ATPase of human skin fibroblasts. Ecotoxicol Environ Saf. 1991 Feb;21(1):38-46.

However, when the detergent sodium dodecyl benzenesulfonate was combined with the herbicide 2,4-dichlorophenoxyacetate (2,4-D), the toxic effect of 2,4-D on the Na+/K (+)-ATPase decreased considerably.
8(0,0,1,3) Details
16806958 Fukuda A, Tanaka Y: Effects of ABA, auxin, and gibberellin on the expression of genes for vacuolar H+ -inorganic pyrophosphatase, H+ -ATPase subunit A, and Na+/H+ antiporter in barley. Plant Physiol Biochem. 2006 May-Jun;44(5-6):351-8. Epub 2006 Jun 16.

Furthermore, treatment with ABA and 2,4-D increased Na (+)/H (+) antiport activity and proton-translocating activities by H (+)-PPase and H (+)-ATPase in tonoplast vesicles, and treatment with ABA also increased the amount of V-PPase protein of tonoplast vesicles.
8(0,0,1,3) Details
15056896 Muller RH, Babel W: Delftia acidovorans MC1 resists high herbicide concentrations--a study of nutristat growth on (RS)-2-(2,4-Dichlorophenoxy) propionate and 2,4-dichlorophenoxyacetate. Biosci Biotechnol Biochem. 2004 Mar;68(3):622-30.

Delftia acidovorans MC1 was continuously cultivated under nutristat conditions with elevated concentrations of the herbicides (RS)-2-(2,4-dichlorophenoxy) propionate [(RS)-2,4-DP] and 2,4-dichlorophenoxyacetate (2,4-D).
7(0,0,1,2) Details
10652787 Muller RH, Jorks S, Kleinsteuber S, Babel W: Comamonas acidovorans strain MC1: a new isolate capable of degrading the chiral herbicides dichlorprop and mecoprop and the herbicides 2,4-D and MCPA. Microbiol Res. 1999 Dec;154(3):241-6.
6(0,0,1,1) Details
8000010 Harms K, Wohner RV, Schulz B, Frommer WB: Isolation and characterization of P-type H (+)-ATPase genes from potato. Plant Mol Biol. 1994 Nov;26(3):979-88.

Only 2,4-D and prolonged periods of darkness lead to a slight reduction in mRNA levels.
4(0,0,0,4) Details
232748 Muhlrad A, Friedman M: Myosin changes in experimental 2,4-dichlorophenoxyacetate myopathy. . Muscle Nerve. 1978 Nov-Dec;1(6):471-8.

Young rats treated with 10 to 14 daily injections of 2,4-dichlorophenoxyacetate (2,4-D) developed a myopathy mainly involving fast muscles.
Alkylation of thiols by N-ethylmaleimide (NEM) induced an increase of Ca2+-activated ATPase that was higher in normal than in myopathic myosin.
2(0,0,0,2) Details
17379940 Hicks-Berger C, Morre DJ: Inside-out but not right side-out plasma membrane vesicles from soybean enlarge when treated with ATP + 2,4-D as determined by electron microscopy and light scattering: evidence for involvement of a plasma membrane AAA-ATPase. Biofactors. 2006;28(2):91-104.

The concept that the location of an AAA-ATPase associated with the plant plasma membrane may be indicative of a functional relationship to growth or cell enlargement by analogy with roles in physical membrane displacements as proposed for AAA-ATPases associated with internal membranes was tested.
2(0,0,0,2) Details
10559217 Pritchard JB, Sweet DH, Miller DS, Walden R: Mechanism of organic anion transport across the apical membrane of choroid plexus. J Biol Chem. 1999 Nov 19;274(47):33382-7.

Brush border membrane vesicles were enriched in Na (+),K (+)-ATPase (20-fold; an apical marker in CP) and demonstrated specific, sodium-coupled transport of proline, glucose, and glutarate.
Vesicular uptake of the anionic herbicide 2, 4-dichlorophenoxyacetic acid (2,4-D) was markedly stimulated by an inward sodium gradient but only in the presence of glutarate, indicating the presence of apical dicarboxylate/organic anion exchange.
1(0,0,0,1) Details
9699988 Morre DJ, Peter AD, Morre DM, Van Alstine JM: Effect of centrifugation on separation by aqueous two-phase partition of an early and late endosome model using inside-out plasma membrane vesicles from plants. J Chromatogr B Biomed Sci Appl. 1998 Jun 26;711(1-2):195-201.

Endosome subpopulations were resolved readily by preparative free-flow electrophoresis where acidification of the interiors of late endosomes occurred upon addition of ATP to activate a proton translocating ATPase.
A latent, 2,4-dichlorophenoxyacetic acid (2,4-D)-(auxin)-stimulated NADH:protein disulfide reductase measured spectrophotometrically was used as an enzymatic marker for both populations of inside-out vesicles.
1(0,0,0,1) Details
17568655 Fanous A, Weiland F, Luck C, Gorg A, Friess A, Parlar H: A proteome analysis of Corynebacterium glutamicum after exposure to the herbicide 2,4-dichlorophenoxy acetic acid (2,4-D). Chemosphere. 2007 Aug;69(1):25-31. Epub 2007 Jun 12.

The last induced protein was the ABC type transport system (Cg2695, ATPase component).
1(0,0,0,1) Details
15832368 Teixeira MC, Santos PM, Fernandes AR, Sa-Correia I: A proteome analysis of the yeast response to the herbicide 2,4-dichlorophenoxyacetic acid. Proteomics. 2005 May;5(7):1889-901.

To gain insights into the mechanisms of adaptation to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), we have identified variations in protein expression level in the eukaryotic experimental model Saccharomyces cerevisiae exposed to herbicide aggression, based on two-dimensional gel electrophoresis.
The increased level of other enzymes involved in protein (Cdc48p) and mRNA (Dcp1p) degradation, in carbohydrate metabolism (Eno1p, Eno2p and Glk1p) and in vacuolar H (+)-ATPase (V-ATPase) function (Vma1p and Vma2p, two subunits of the peripheral catalytic sector) was also registered.
1(0,0,0,1) Details
8048053 Palmeira CM, Moreno AJ, Madeira VM: Interactions of herbicides 2,4-D and dinoseb with liver mitochondrial bioenergetics. Toxicol Appl Pharmacol. 1994 Jul;127(1):50-7.

Additionally, it increased the rate of state 4 oxygen consumption, stimulated ATPase activity, induced permeabilization of membrane mitochondria to H+, and depressed delta psi.
1(0,0,0,1) Details
2148478 Arguello JM, Evangelista de Duffard AM, Duffard RO: Ca2+ homeostasis alterations induced by 2,4-dichlorophenoxyacetic butyl ester and 2,4-dichlorophenoxyacetic acid on avian skeletal muscle. Biochem Pharmacol. 1990 Dec 1;40(11):2441-8.

Fertilized hen eggs were treated externally with 2,4-dichlorophenoxyacetic butyl ester (2,4-D b.e.) (3.1 mg/egg) before the start of the incubation.
Actomyosin and sarcoplasmic reticulum adenosine triphosphatase (ATPase) activities from leg and complexus muscles of chicks hatched from treated eggs were measured.
1(0,0,0,1) Details
8553371 Bergesse JR, Balegno HF: 2,4-Dichlorophenoxyacetic acid influx is mediated by an active transport system in Chinese hamster ovary cells. Toxicol Lett. 1995 Nov 15;81(2-3):167-73.


The influx of 2,4-dichlorophenoxyacetic acid (2,4-D) into Chinese hamster ovary (CHO) cells was studied.
0(0,0,0,0) Details
15466552 Muller TA, Byrde SM, Werlen C, van der Meer JR, Kohler HP: Genetic analysis of phenoxyalkanoic acid degradation in Sphingomonas herbicidovorans MH. Appl Environ Microbiol. 2004 Oct;70(10):6066-75.


We report the isolation of several genes involved in dichlor- and mecoprop degradation from the alphaproteobacterium Sphingomonas herbicidovorans MH and propose that the degradation proceeds analogously to that previously reported for 2,4-dichlorophenoxyacetic acid (2,4-D).
0(0,0,0,0) Details
1447481 Janik F, Wolf HU: The Ca (2+)-transport-ATPase of human erythrocytes as an in vitro toxicity test system--acute effects of some chlorinated compounds. J Appl Toxicol. 1992 Oct;12(5):351-8.

Investigations to determine the inhibitory activity on the Ca (2+)-transport-ATPase of human erythrocyte membranes were performed with various compounds of toxicological significance, mostly chlorinated and mainly used as biocides, such as phenol, 4-chlorophenol, 2,4-dichlorophenol, 2,6-dichlorophenol, 3,4-dichlorophenol, 2,3,4-trichlorophenol, 2,3,4,5-tetrachlorophenol, pentachlorophenol (PCP), captan, folpet, captafol, (+)-camphene, toxaphene, dichlorodiphenyltrichloroethane (DDT), lindane, endrin, dieldrin, alpha-endosulfan, beta-endosulfan, paraquat, diallate, 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T).
34(0,1,1,4) Details