Protein Information

Name casein
Synonyms Alpha; Alpha s1 casein; Alpha s1 casein precursor; CASA; CSN 1; CSN1; CSN1S1; Casein…

Compound Information

Name 2,4-D
CAS 2-(2,4-dichlorophenoxy)acetic acid

Reference List

PubMed Abstract RScore(About this table)
9570830 Josten A, Meusel M, Spener F: Microbial transglutaminase-mediated synthesis of hapten-protein conjugates for immunoassays. Anal Biochem. 1998 May 1;258(2):202-8.

Different procedures for the synthesis and the immobilization of these 2,4-D-casein conjugates were evaluated, comprising (i) a batch procedure, (ii) coupling of 2,4-D to an already immobilized layer of casein, and (iii) a method for simultaneous immobilization and conjugation.
112(1,2,2,2) Details
20112812 Rajab R, Rajan SS, Satheesh LS, Harish SR, Sunukumar SS, Sandeep BS, Mohan TC, Murugan K: Hypersensitive response of Sesamum prostratum Retz. elicitated by Fusarium oxysporum f. sesame (Schelt) Jacz Butler. Indian J Exp Biol. 2009 Oct;47(10):834-8.

Aim of this study was to investigate the intensity and timing of the ROS formation, lipid peroxidation and expression of antioxidant enzymes as initial responses of calli of Sesamum prostratum (SP) against Fusarium oxysporum f. sesame crude toxin metabolite of varying concentrations. 2,4 dichlorophenoxy acetic acid (2,4-D) / coconut milk combinations were found to be more efficient among different hormonal regimes (2,4 -D, 2,4-D/casein hydrosylate and 2,4-D/ coconut milk).
31(0,1,1,1) Details
15197305 Meusel M: Synthesis of hapten-protein conjugates using microbial transglutaminase. Methods Mol Biol. 2004;283:109-23.

Methods for the synthesis of 2,4-D-casein conjugates (2,4-diclorophenoxyacetic acid, a herbicide) are presented.
31(0,1,1,1) Details
15969112 Jin H, Jia JF, Hao JG: [Protoplast culture and plant regeneration of the methionine resistant variant of Astragalus melilotoides Pall]. Sheng Wu Gong Cheng Xue Bao. 2004 Mar;20(2):221-6.

The results show that agarose-beads culture method, KM8p medium supplemented with 1.0 mg/L 2,4-D, 0.5mg/L 6BA, 0.3 mol/L mannitol, 2% (W/V) sucrose and 500 mg/L casein hydrolysate at a plating density of 3 x 10 (5)/mL are the appropriate conditions for protoplast division of the methionine resistant cell line.
6(0,0,1,1) Details
16944592 Zhang GN, Wang YH, Wang XR, He T, Hao JG, Jia JF: [Protoplast culture and plant regeneration of the methionine resistant variant of Astragalus cicer L]. Fen Zi Xi Bao Sheng Wu Xue Bao. 2006 Jun;39(3):191-8.

Sustained cell divisions and colony formation from the protoplasts of the methionine resistant cell line of Astragalus cicer L. were obtained by a DPD medium containing 2.0 mg/L 2,4- dichlorophenoxyacetic acid (2,4-D), 0.2 mg/L 6 -benzylaminopurine (6-BA), 0.3 mol/L mannitol, 200 mg/L casein hydrolysate and 2% (W/V) sucrose at a plating density of 2x10 (5) /ml.
6(0,0,1,1) Details
16862300 Franco ET, Gavioli LB, Ferreira AG: In vitro regeneration of Didymopanax morototoni. Braz J Biol. 2006 May;66(2A):455-62.

In the first step, woody plant medium (WPM) with casein hydrolysate (250 mgL-1 ) and 2,4-D (1.0 and 5.0 mgL-1) were used combined with kinetin (0.1 and 1.0 mgL-1).
6(0,0,1,1) Details
12789423 Yemets AI, Klimkina LA, Tarassenko LV, Blume YB: Efficient callus formation and plant regeneration of goosegrass [Eleusine indica (L.) Gaertn.]. Plant Cell Rep. 2003 Feb;21(6):503-10. Epub 2002 Dec 14.

The optimum medium for inducing morphogenic calli consisted of N6 basal salts and B5 vitamins supplemented with 1-2 mg l (-1) 2,4-dichlorophenoxyacetic acid (2,4-D), 2 mg l (-1) glycine, 100 mg l (-1) asparagine, 100 mg l (-1) casein hydrolysate, 30 g l (-1) sucrose and 0.6% agar, pH 5.7.
6(0,0,1,1) Details
15959731 Ramakrishnan K, Gnanam R, Sivakumar P, Manickam A: In vitro somatic embryogenesis from cell suspension cultures of cowpea [Vigna unguiculata (L.) Walp]. Plant Cell Rep. 2005 Oct;24(8):449-61. Epub 2005 Jun 16.

Primary leaf-derived, embryogenic calli initiated in MMS [MS salts (Murashige and Skoog 1962) with B5 (Gamborg et al. 1968) vitamins] medium containing 2,4-Dichlorophenoxyacetic acid (2,4-D), casein hydrolysate (CH), and L: -Glutamic acid-5-amide (Gln).
6(0,0,1,1) Details
9581525 Jha S, Sanyal D, Ghosh B, Jha TB: Improved taxol yield in cell suspension culture of Taxus wallichiana (Himalayan yew). Planta Med. 1998 Apr;64(3):270-2.

Cell line NC110 derived from needle leaf of a 40-year-old tree growing in Darjeeling Himalayas produced 0.018% taxol in B5 basal medium supplemented with 2,4-D (2 mg/l), kinetin (0.5 mg/l), and casein hydrolysate (0.5%).
6(0,0,1,1) Details
7765304 Fett-Neto AG, Melanson SJ, Sakata K, DiCosmo F: Improved growth and taxol yield in developing calli of Taxus cuspidata by medium composition modification. Biotechnology (N Y). 1993 Jun;11(6):731-4.

Several sequential modifications were made to the basal B5 medium, which included addition and/or variation in the concentration of sucrose, B5 organic supplements, gibberellic acid, 36 combinations of 2,4-D/kinetin ratios, media salts and organic supplements, phenylalanine, casein hydrolysate and medium pH.
6(0,0,1,1) Details
11037948 Wang P, Ou S, Peilin C: Optimization of conditions for safflower cell culture and accumulation of cellicolous product tocopherols. Chin J Biotechnol. 1999;15(4):231-7.

When 0.1% casein was added into MS medium, and 2,4-D concentration was adjucted to 0.30 mg/L and 6-BA to 1.80 mg/L, alpha-, beta- and gamma-forms of tocopherol reached 4.1706, 0.3120 and 0.1650 microg/g fresh calli, respectively.
6(0,0,1,1) Details
16850870 Li L, Zhang CR: Production of puerarin and isoflavones in cell suspension cultures of Pueraria lobata (Willd.): effects of medium supplementation with casein hydrolysate and coconut milk. J Environ Biol. 2006 Jan;27(1):21-6.

The optimum medium for cell cultures of leaves of P. lobata seedlings was B5 liquid medium supplemented with 2% sucrose, 1.0 mg l (-1) 2,4-D, 1.0 mg l (-1) NAA, 0.5 mg l (-1) kinetin and 20 mg l (-1) CH.
2(0,0,0,2) Details
16493521 De Silva AE, Kadir MA, Aziz MA, Kadzimin S: Proliferation potential of 18-month-old callus of Ananas comosus L. cv. ScientificWorldJournal. 2006 Feb 17;6:169-75.

Medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) was inferior to NAA, while b-naphthoxy acetic acid (BNOA) and p-chlorophenoxy acetic acid (4-CPA) were not effective in proliferating 18-months old callus.
Addition of casein hydrolysate and coconut water to NAA supplemented medium showed better proliferation and production of callus.
1(0,0,0,1) Details
8225416 Raval M, Chattoo BB: Role of media constituents and proline in callus growth, somatic embryogenesis and regeneration of Oryza sativa cv Indica. Indian J Exp Biol. 1993 Jul;31(7):600-3.


Addition of 2,4-D to MS medium at 2.5 mg/l resulted in 100% callus induction.
0(0,0,0,0) Details