Protein Information

Name C18
Synonyms B1; D1; BBS 9; BBS9; BBS9 GENE; Bardet Biedl syndrome 9 protein; C18; PTH responsive osteosarcoma B1 protein…

Compound Information

Name acrolein
CAS 2-propenal

Reference List

PubMed Abstract RScore(About this table)
18422332 da Silva TO, Pereira PA: Influence of time, surface-to-volume ratio, and heating process (continuous or intermittent) on the emission rates of selected carbonyl compounds during thermal oxidation of palm and soybean oils. J Agric Food Chem. 2008 May 14;56(9):3129-35. Epub 2008 Apr 19.

The CC were collected and derivatized onto silica C18 cartridges impregnated with an acid 2,4-dinitrophenylhidrazine solution, followed by extraction with acetonitrile and analysis by HPLC-UV and, in some cases, HPLC-MS with electrospray ionization.
Among the CC quantified, namely, acetaldehyde, acrolein, propanal, butanal, hexanal, 2-heptenal, and 2-octenal, acrolein was the main emission in both oils and all S/ V ratios, followed by hexanal and 2-heptenal.
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18481328 de Carvalho AB, Kato M, Rezende MM, de P Pereira PA, de Andrade JB: Determination of carbonyl compounds in the atmosphere of charcoal plants by HPLC and UV detection. J Sep Sci. 2008 Jun;31(10):1686-93.

A chromatographic quantification method with two different mobile phases (elution conditions 1 and 2) was developed to determine carbonyl compounds (CCs) in air samples collected from charcoal production workplaces, using C18 cartridges coated with 2,4-dinitrophenylhydrazine (DNPHi).
In 16 min, elution condition 1 successfully separated and quantified the DNPHo of 14 CC including acetaldehyde, acrolein, formaldehyde, and furfural, and estimated the sum of C4 isomers, butanal-isobutanal-butanone.
1(0,0,0,1) Details
10452807 Huang Z, Waxman DJ: High-performance liquid chromatographic-fluorescent method to determine chloroacetaldehyde, a neurotoxic metabolite of the anticancer drug ifosfamide, in plasma and in liver microsomal incubations. Anal Biochem. 1999 Aug 15;273(1):117-25.

The derivatization mixture is directly injected onto a C18 HPLC column and is monitored with a fluorescence detector.
By coupling this method with a recently described HPLC-fluorescent method to determine acrolein, a cytochrome P450 metabolite of IFA formed during the activation of the drug by 4-hydroxylation, the two major, alternative P450-catalyzed pathways of IFA metabolism can be monitored from the same plasma samples or liver microsomal incubations and the partitioning of drug between these two pathways thereby quantitated.
1(0,0,0,1) Details
10399334 Sakuragawa A, Yoneno T, Inoue K, Okutani T: Trace analysis of carbonyl compounds by liquid chromatography-mass spectrometry after collection as 2,4-dinitrophenylhydrazine derivatives. J Chromatogr A. 1999 Jun 4;844(1-2):403-8.

After the carbonyl compounds are collected using a Waters Sep-Pak C18 cartridge column with-impregnated DNPH on octadecylsilica, they are eluted by acetonitrile as carbonyl-DNPH derivatives.
The four derivatives (formaldehyde-, acetaldehyde-, acrolein- and acetone-DNPH) were eluted within 7 min with acetonitrile-water (60:40, v/v) as the mobile phase.
1(0,0,0,1) Details
20188957 de M Ochs S, Fasciotti M, Barreto RP, de Figueiredo NG, Albuquerque FC, Massa MC, Gabardo I, Pereira Netto AD: Optimization and comparison of HPLC and RRLC conditions for the analysis of carbonyl-DNPH derivatives. Talanta. 2010 Apr 15;81(1-2):521-9. Epub 2010 Jan 4.

Chromatograms run with a Zorbax Eclipse Plus C18 (50 mm x 2.1 mm x 1.8 microm) column and a mobile phase composed of isopropanol, methanol, tetrahydrofuran and water led to the best separation conditions considering reduced analysis time (approximately 6 min per run), solvent consumption rate (approximately 2 mL per run) and resolution of propanone, acrolein and propionaldehyde hydrazones.
33(0,1,1,3) Details
11954678 Grosjean D, Grosjean E: Airborne carbonyls from motor vehicle emissions in two highway tunnels. Res Rep Health Eff Inst. 2002 Jan;(107):57-78; discussion 79-92.

At the Tuscarora Mountain Tunnel, the LD emission factor was 94.9 mg/L for total measured carbonyls; the ten most abundant carbonyls were formaldehyde, acetone, acetaldehyde, heptanal, crotonaldehyde, 2-butanone, propanal, acrolein, methacrolein, and benzaldehyde.
Co-located samples, one collected using a DNPH-coated silica gel cartridge and the other using a DNPH-coated C18 cartridge, were collected downstream of KI oxidant scrubbers at the inlet and outlet of the Caldecott Tunnel.
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12661015 Andreoli R, Manini P, Corradi M, Mutti A, Niessen WM: Determination of patterns of biologically relevant aldehydes in exhaled breath condensate of healthy subjects by liquid chromatography/atmospheric chemical ionization tandem mass spectrometry. Rapid Commun Mass Spectrom. 2003;17(7):637-45.

Malondialdehyde (MDA), acrolein, alpha,beta-unsaturated hydroxylated aldehydes [namely 4-hydroxyhexenal (4-HHE) and 4-hydroxynonenal (4-HNE)], and saturated aldehydes (n-hexanal, n-heptanal and n-nonanal) were measured in EBC after derivatization with 2,4-dinitrophenylhydrazine (DNPH).
DNPH derivatives were separated on a C18 column using variable proportions of 20 mM aqueous acetic acid and methanol.
1(0,0,0,1) Details
6530448 Wisneski HH, Yates RL, Davis HM: High-performance liquid chromatographic-fluorometric determination of cinnamaldehyde in perfume, cologne and toilet water. J Chromatogr. 1984 Dec 28;317:421-6.


Aliquots of the fluorophore solution are analyzed on a reversed-phase C18 HPLC column by using a buffered tetrahydrofuran-water eluent.
1(0,0,0,1) Details
12516465 Luo JP, Zhang ZS, Tian C, Li XM: [Determination of cinnamaldehyde in Guizhi by HPLC] . Zhongguo Zhong Yao Za Zhi. 2000 Sep;25(9):544-5.


METHOD: Cinnamaldehyde was separated by a Kromasil C18 column (4.6 mm x 200 mm) with a mobile phase of acetonitrile-0.1% phosphoric acid solution (48:52) and an UV detection wavelength of 285 nm.
1(0,0,0,1) Details
1757421 Lane RH, Smathers JL: Monitoring aldehyde production during frying by reversed-phase liquid chromatography. J Assoc Off Anal Chem. 1991 Nov-Dec;74(6):957-60.

Acrolein (2-propenal) and other low molecular weight aldehydes (LMWAs) formed by degradation of the frying medium (triglycerides) were monitored by liquid chromatography (LC) during preparation of fried items.
The hydrazones are separated and quantified on a C18 reversed-phase column with acetonitrile-water as the mobile phase.
1(0,0,0,1) Details
10995112 Wiesenthal K, Jehlar A, Que Hee SS, Wiesenthal K, Jehlar A, Que Hee SS: Synthesis of the O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine oximes of selected carbonyl compounds and their determination by liquid chromatography with ultraviolet detection. J AOAC Int. 2000 Jul-Aug;83(4):859-69.

A new C18 reverse-phase LC method with UVD at 200 nm and acetonitrile-water in both the isocratic and gradient-elution modes was then developed to sensitively resolve a mixture of 13 pure PFBHA O-oximes.
Vapors of n-valeraldehyde and acrolein generated in gas bags at concentrations near occupational guidelines were also sampled, desorbed, and then determined by either isocratic or gradient elution at 200 or 254 nm within 30-45 min.
1(0,0,0,1) Details
8290470 al-Rawithi S, el-Yazigi A, Nicholls PJ: Determination of acrolein in urine by liquid chromatography and fluorescence detection of its quinoline derivative. Pharm Res. 1993 Nov;10(11):1587-90.

The derivative (7-OH quinoline; DER) and the internal standard (quinine-bisulfate; IS) were separated on a 10-micron particle, 8 mm x 10-cm C18 cartridge in conjunction with a radial compression system using a mixture of 0.05 M dibasic ammonium phosphate solution (pH 2.5):acetonitrile:methanol (92:6:2) at a flow rate of 3 mL/min as a mobile phase.
1(0,0,0,1) Details
10755368 Paci A, Rieutord A, Guillaume D, Traore F, Ropenga J, Husson HP, Brion F: Quantitative high-performance liquid chromatographic determination of acrolein in plasma after derivatization with Luminarin 3. J Chromatogr B Biomed Sci Appl. 2000 Mar 10;739(2):239-46.

Chromatographic conditions involved a C18 RP column Uptisphere and a gradient elution system to optimize resolution and time analysis.
1(0,0,0,1) Details