Protein Information

Name CD34
Synonyms CD34; CD34 antigen; CD34 molecule; CD34F; HPCA 1; HPCA1; Hematopoietic progenitor cell antigen; Hematopoietic progenitor cell antigen CD34…

Compound Information

Name sulfoxide
CAS 5-[2-(octylsulfinyl)propyl]-1,3-benzodioxole

Reference List

PubMed Abstract RScore(About this table)
18179677 Reich-Slotky R, Colovai AI, Semidei-Pomales M, Patel N, Cairo M, Jhang J, Schwartz J: Determining post-thaw CD34+ cell dose of cryopreserved haematopoietic progenitor cells demonstrates high recovery and confirms their integrity. Vox Sang. 2008 May;94(4):351-7. Epub 2008 Jan 2.

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18700465 Balint B, Ljubenov M, Stamatovic D, Todorovic M, Pavlovic M, Ostojic G, Jocic M, Trkuljic M: Stem cell harvesting protocol research in autologous transplantation setting: large volume vs. conventional cytapheresis. Vojnosanit Pregl. 2008 Jul;65(7):545-51.


The CD34+ cell surface antigen was investigated by the EPICS XL-MCL device (Coulter, Germany).
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17408467 Alcantara O, Boldt DH: Iron deprivation blocks multilineage haematopoietic differentiation by inhibiting induction of p21 (WAF1/CIP1). Br J Haematol. 2007 May;137(3):252-61.


To determine if the same requirement holds true for differentiation in other cell lineages and for primary human CD34 (+) bone marrow precursor cells, we induced granulocyte differentiation by treating HL-60 cells with dimethyl sulphoxide, and erythroid or megakaryocytic differentiation by treating K562 cells with butyrate or phorbol myristate acetate, respectively.
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18682250 Miura J, Minegishi M, Itoh T, Kitaura T, Fukawa N, Takahashi H, Suzuki A, Kudo Y, Narita A, Sato Y, Suzuki M, Wada Y, Takeyama Y, Watanabe T, Tsuchiya S: Quality evaluation of umbilical cord blood progenitor cells cryopreserved with a small-scale automated liquid nitrogen system. Cryobiology. 2008 Oct;57(2):178-81. Epub 2008 Jul 19.


After thawing the units, the viability and recovery of cells, as well as the recovery rate of hematopoietic progenitor cells (HPCs) such as CD34+ cells, colony-forming unit-granulocyte-macrophage (CFU-GM), and total CFU were analyzed.
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19392777 Liseth K, Ersvaer E, Abrahamsen JF, Nesthus I, Ryningen A, Bruserud O: Long-term cryopreservation of autologous stem cell grafts: a clinical and experimental study of hematopoietic and immunocompetent cells. Transfusion. 2009 Apr 20.


STUDY DESIGN AND METHODS: Cryopreserved PBPCs were prepared with different concentrations of dimethyl sulfoxide (DMSO; 2, 4, 5, and 10%) and stored for at least 5 years before the recovery of CD34+ cells and various T- and natural killer (NK)-cell subsets were analyzed by flow cytometry.
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18298597 Tijssen MR, Woelders H, de Vries-van Rossen A, van der Schoot CE, Voermans C, Lagerberg JW: Improved postthaw viability and in vitro functionality of peripheral blood hematopoietic progenitor cells after cryopreservation with a theoretically optimized freezing curve. Transfusion. 2008 May;48(5):893-901. Epub 2008 Feb 21.


CD34+-selected and -unselected PBSCs were cryopreserved with the current or the new freezing curves.
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20138169 Son JH, Heo YJ, Park MY, Kim HH, Lee KS: Optimization of cryopreservation condition for hematopoietic stem cells from umbilical cord blood. Cryobiology. 2010 Feb 4.


The conditions for cryopreservation of CD34 (+) hematopoietic stem cells (HSC) from umbilical cord blood (UCB) were optimized with a new cryo-medium containing 10% ethylene glycol (EG) and 2% dimethyl sulfoxide (Me (2) SO) using a controlled-rate freezing (CRF) method.
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18401596 Strakova Z, Livak M, Krezalek M, Ihnatovych I: Multipotent properties of myofibroblast cells derived from human placenta. . Cell Tissue Res. 2008 Jun;332(3):479-88. Epub 2008 Apr 10.


Flow cytometry of HuF has detected the presence of CD34, a marker of the hematopoietic stem cell lineage, and an absence of CD10, CD11b/Mac, CD14, CD45, and HLA type II.
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19878061 Akkok CA, Liseth K, Hervig T, Ryningen A, Bruserud O, Ersvaer E: Use of different DMSO concentrations for cryopreservation of autologous peripheral blood stem cell grafts does not have any major impact on levels of leukocyte- and platelet-derived soluble mediators. Cytotherapy. 2009;11(6):749-60.


Cryopreservation using 5% instead of 10% DMSO improves CD34 (+) cell viability and therefore we investigated whether using less DMSO had favorable outcomes on leukocyte viability and levels of various soluble mediators in the graft supernatant.
1(0,0,0,1) Details
19040412 Lecchi L, Perego L, Garcea F, Ratti I, Brasca M, Dotti D, Cimoni S, Pezzali I, Celeste T, Giovanelli S, Butti B, De Fazio N, Lopa R, Rebulla P: Ten-year quality control of a semiautomated procedure of cord blood unit volume reduction. Transfusion. 2009 Mar;49(3):563-9. Epub 2008 Nov 24.


Total nucleated cells (TNCs) were counted before and after processing in the 4311 units banked from 1998 through 2007, whereas CD34+ cells and colony-forming units-granulocyte-macrophage (CFU-GM) were counted in 420 random units from 2001 through 2007.
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17786608 Kim MH, Kim I, Kim SH, Jung MK, Han S, Lee JE, Nam JS, Lee SK, Bang SI: Cryopreserved human adipogenic-differentiated pre-adipocytes: a potential new source for adipose tissue regeneration. Cytotherapy. 2007;9(5):468-76.


In addition, CD34-positive endothelial cells were detected in implants.
1(0,0,0,1) Details
19929473 Solves P, Mirabet V, Blanquer A, Delgado-Rosas F, Planelles D, Andrade M, Carbonell-Uberos F, Soler MA, Roig R: A new automatic device for routine cord blood banking: critical analysis of different volume reduction methodologies. Cytotherapy. 2009;11(8):1101-7.


In the multivariate analysis, when analyzing all cases, the BC volume set significantly influenced TNC, CD34+ and lymphocyte recoveries and RBC depletion (P <0.001).
1(0,0,0,1) Details
19551541 Akkok CA, Liseth K, Hervig T, Ryningen A, Bruserud O, Ersvaer E: Use of different DMSO concentrations for cryopreservation of autologous peripheral blood stem cell grafts does not have any major impact on levels of leukocyte- and platelet-derived soluble mediators. Cytotherapy. 2009 Jun 23:1-12.


Cryopreservation using 5% instead of 10% DMSO improves CD34 (+) cell viability and therefore we investigated whether using less DMSO had favorable outcomes on leukocyte viability and levels of various soluble mediators in the graft supernatant.
1(0,0,0,1) Details
17606452 Galmes A, Gutierrez A, Sampol A, Canaro M, Morey M, Iglesias J, Matamoros N, Duran MA, Novo A, Bea MD, Galan P, Balansat J, Martinez J, Bargay J, Besalduch J: Long-term hematological reconstitution and clinical evaluation of autologous peripheral blood stem cell transplantation after cryopreservation of cells with 5% and 10% dimethylsulfoxide at -80 degrees C in a mechanical freezer. Haematologica. 2007 Jul;92(7):986-9.


Factors influencing a faster hematological recovery were infusion of more than 2.7x10 (6)/Kg of CD34+ cells, 10% DMSO cryopreservation and G-CSF.
1(0,0,0,1) Details
18220853 Bakken AM: Cryopreserving human peripheral blood progenitor cells. Curr Stem Cell Res Ther. 2006 Jan;1(1):47-54.


In recent studies done with PBPC cells cryopreserved with 5%, 4% and 2% DMSO, using 10% DMSO as a reference control, CD34+ cells were investigated for preservation of viability, apoptosis, and necrosis.
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17714421 Iannalfi A, Bambi F, Tintori V, Lacitignola L, Bernini G, Mariani MP, Sanvito MC, Pagliai F, Brandigi F, Muscarella E, Tapinassi F, Faulkner L: Peripheral blood progenitor uncontrolled-rate freezing: a single pediatric center experience. Transfusion. 2007 Dec;47(12):2202-6. Epub 2007 Aug 21.


All grafts contained at least 2 x 10 (6) per kg unselected CD34+ cells, enumerated before freezing.
1(0,0,0,1) Details
18765238 Petrenko YA, Jones DR, Petrenko AY: Cryopreservation of human fetal liver hematopoietic stem/progenitor cells using sucrose as an additive to the cryoprotective medium. Cryobiology. 2008 Dec;57(3):195-200. Epub 2008 Aug 14.


For the determination of the number and survival of hematopoietic progenitors present, cells were stained with CD34 (FITC) and 7-AAD, and analyzed by flow cytometry.
1(0,0,0,1) Details
19912586 McCullough J, Haley R, Clay M, Hubel A, Lindgren B, Moroff G: Long-term storage of peripheral blood stem cells frozen and stored with a conventional liquid nitrogen technique compared with cells frozen and stored in a mechanical freezer. Transfusion. 2009 Nov 13.


Cells were stored for 5 years during which total nucleated cells (TNCs), cell viability, CD34+ cell content, and colony-forming unit-granulocyte-macrophage content were determined.
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19041066 Ozdemir E, Akgedik K, Akdogan S, Kansu E: The lollipop with strawberry aroma may be promising in reduction of infusion-related nausea and vomiting during the infusion of cryopreserved peripheral blood stem cells. Biol Blood Marrow Transplant. 2008 Dec;14(12):1425-8.


The 2 groups were comparable with respect to age, sex, diagnosis, stem cell collection methods, conditioning regimens administered, total mononuclear cell dose infused, number of total nucleated cells (TNCs) infused, number of CD34+ cells infused, number of bags infused, total volume infused, amount of dimethylsulfoxide (DMSO), and infusion rate.
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